Method for the detection and possible quantification of activity of at least one protease inhibitor in a sample to be analyzed, as well as a method for the determination of the (residual) activity of one (or more) discrete (iso)type(s) protease inhibitor(s) and application of the method for the determination of the total (residual) activity of a sample, if necessary after processing such as a process for the elimination or inactivation of protease inhibitor(s)

A. van Amerongen (Inventor), M.M.T. Meijer (Inventor)

    Research output: PatentOther research output

    Abstract

    The present invention relates to a method for detecting and, if necessary, quantifying the activity of at least one protease inhibitor in a mixture to be analysed, where a) the mixture to be analysed or a sample thereof is brought into contact with at least one protease for the protease inhibitor to be detected, with which protease the active form of the protease inhibitors undergoes complex formation to a (protease-(protease inhibitor)) complex and with which protease still inactive free protease inhibitor still binds already complexed protease inhibitor, b) the amount of (protease-(protease inhibitor)) complex formed in step a is determined. The invention also relates to the application of this method for determining the (residual) activity of one (or more) separate (iso)type(s) of protease inhibitor(s) and application of this method for determining the total (residual) activity of a mixture, possibly after a processing process such as a process for the removal or inactivation of protease inhibitor(s).
    Original languageEnglish
    Patent numberNL9301134
    Publication statusPublished - 16 Jan 1995

    Fingerprint Dive into the research topics of 'Method for the detection and possible quantification of activity of at least one protease inhibitor in a sample to be analyzed, as well as a method for the determination of the (residual) activity of one (or more) discrete (iso)type(s) protease inhibitor(s) and application of the method for the determination of the total (residual) activity of a sample, if necessary after processing such as a process for the elimination or inactivation of protease inhibitor(s)'. Together they form a unique fingerprint.

  • Cite this