Three institutes worked together on the development and improvement of methods for the analysis of polybrominated diphenylethers (PBDEs) in sediments and biota. The methods developed consist of a Soxhlet, Ultra Turrax* (biota) or shake-flask extraction (sediment) using hexane (or pentane)/acetone solvent mixtures, clean-up by alumina column chromatography or by gel permeation chromatography, further clean-up by silica gel column chromatography and treatment with sulphuric acid. Several alternatives for extraction and clean-up yielded similar results. The final determination is performed by GC/NCI-MS in the selected ion mode with m/z values of both Br isotopes (79 and 81 occurring in a 1:1 ratio). For decaBDE the fragments m/z 486.7 and 488.7 were also scanned. The coefficients of variation obtained with this method are between 10 and 35␏or individual congeners, and comply at least with the international state-of-the-art for this type of analysis.
de Boer, J., Allchin, C., Law, R., Zegers, B., & Boon, J. P. (2001). Method for the analysis of polybrominated diphenylethers in sediments and biota. TrAC : Trends in Analytical Chemistry, 20(10), 591-599. https://doi.org/10.1016/S0165-9936(01)00097-8