Abstract
A type lll-B system from Haliangium ochraceum contains two caspase-like proteases, SAVED-CHAT and PCaspase (prokaryotic caspase). Cyclic tri-adenosine monophosphate (AMP)-induced oligomerization of SAVED-CHAT activates proteolytic activity of the CHAT domains, which specifically cleave and activate PCaspase. Subsequently, activated PCaspase cleaves a multitude of proteins, which results in a strong interference phenotype in vivo in Escherichia coli. This provides for a CRISPR-Cas-based method of detecting a target RNA, whereby detection signalling is triggered via a cascade of caspase-associated proteolytic activities. Polynucleotides, plasmids, vectors and nucleoprotein complexes comprising nucleic acids encoding the components of the system are provided.
Original language | English |
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Patent number | WO2024256537 |
Priority date | 14/06/23 |
Publication status | Published - 19 Dec 2024 |