Methicillin-resistant Staphylococcus pseudintermedius among dogs in the description of novel SCCmec variants

Birgitta Duim, Koen M.H.W. Verstappen, Ruwani S. Kalupahana, Lakmali Ranathunga, Ad C. Fluit, Jaap A. Wagenaar

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Abstract

The presence and genetic characteristics of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Sri Lanka was investigated to add additional insight into global spread, emergence and evolution of MRSP. A total of 234 samples from dogs visiting veterinary clinics were cultured for staphylococci and the genomes of the MRSP isolates were sequenced, to identify resistance genes, the multilocus sequence types (MLST) and spa types. From a questionnaire the history of antimicrobial treatment and patient information was obtained. S. pseudintermedius was isolated from 116/229 samples, eight of these were MRSP. Six MRSP CC45 isolates contained a pseudo-SCC element ΨSCCmec57395. Two isolates belonging to ST429 (CC761) and ST121 (CC121) contained novel variants of the SCCmec Type V(T) element. The elements were designated SCCmecV(T)SL/066, that carried additional transposon-related genes, and SCCmecV(T)SL/154 that carried a type III restriction-modification system, a type 7 ccr gene complex, and a cadA coding sequence. Thirty-seven percent of the dogs received antimicrobial treatment at the time of sampling of which four dogs were MRSP-positive. The proportion of MRSP among S. pseudintermedius is low compared to other countries, despite the fact that in Sri Lanka antimicrobials for treatment of dogs are available over the counter. Important is the finding of novel type V(T) SCCmec elements, which further underlines the high recombination frequency of SCC elements. The ΨSCCmec57395 was found in isolates of CC45, which is the only sequence type of MRSP known to contain this pseudo-cassette.
Original languageEnglish
Pages (from-to)136-141
JournalVeterinary Microbiology
Volume213
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Methicillin Resistance
methicillin
Staphylococcus
Dogs
dogs
Sri Lanka
anti-infective agents
DNA Restriction-Modification Enzymes
Genes
Animal Hospitals
Staphylococcus pseudintermedius
genes
restriction endonucleases
veterinary clinics
Genetic Recombination
sampling
transposons
Therapeutics
History
Genome

Keywords

  • S. pseudintermedius
  • SCCmec variants
  • Sri Lanka
  • ΨSCCmec

Cite this

Duim, B., Verstappen, K. M. H. W., Kalupahana, R. S., Ranathunga, L., Fluit, A. C., & Wagenaar, J. A. (2018). Methicillin-resistant Staphylococcus pseudintermedius among dogs in the description of novel SCCmec variants. Veterinary Microbiology, 213, 136-141. https://doi.org/10.1016/j.vetmic.2017.11.022
Duim, Birgitta ; Verstappen, Koen M.H.W. ; Kalupahana, Ruwani S. ; Ranathunga, Lakmali ; Fluit, Ad C. ; Wagenaar, Jaap A. / Methicillin-resistant Staphylococcus pseudintermedius among dogs in the description of novel SCCmec variants. In: Veterinary Microbiology. 2018 ; Vol. 213. pp. 136-141.
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Duim, B, Verstappen, KMHW, Kalupahana, RS, Ranathunga, L, Fluit, AC & Wagenaar, JA 2018, 'Methicillin-resistant Staphylococcus pseudintermedius among dogs in the description of novel SCCmec variants' Veterinary Microbiology, vol. 213, pp. 136-141. https://doi.org/10.1016/j.vetmic.2017.11.022

Methicillin-resistant Staphylococcus pseudintermedius among dogs in the description of novel SCCmec variants. / Duim, Birgitta; Verstappen, Koen M.H.W.; Kalupahana, Ruwani S.; Ranathunga, Lakmali; Fluit, Ad C.; Wagenaar, Jaap A.

In: Veterinary Microbiology, Vol. 213, 01.01.2018, p. 136-141.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Methicillin-resistant Staphylococcus pseudintermedius among dogs in the description of novel SCCmec variants

AU - Duim, Birgitta

AU - Verstappen, Koen M.H.W.

AU - Kalupahana, Ruwani S.

AU - Ranathunga, Lakmali

AU - Fluit, Ad C.

AU - Wagenaar, Jaap A.

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AB - The presence and genetic characteristics of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Sri Lanka was investigated to add additional insight into global spread, emergence and evolution of MRSP. A total of 234 samples from dogs visiting veterinary clinics were cultured for staphylococci and the genomes of the MRSP isolates were sequenced, to identify resistance genes, the multilocus sequence types (MLST) and spa types. From a questionnaire the history of antimicrobial treatment and patient information was obtained. S. pseudintermedius was isolated from 116/229 samples, eight of these were MRSP. Six MRSP CC45 isolates contained a pseudo-SCC element ΨSCCmec57395. Two isolates belonging to ST429 (CC761) and ST121 (CC121) contained novel variants of the SCCmec Type V(T) element. The elements were designated SCCmecV(T)SL/066, that carried additional transposon-related genes, and SCCmecV(T)SL/154 that carried a type III restriction-modification system, a type 7 ccr gene complex, and a cadA coding sequence. Thirty-seven percent of the dogs received antimicrobial treatment at the time of sampling of which four dogs were MRSP-positive. The proportion of MRSP among S. pseudintermedius is low compared to other countries, despite the fact that in Sri Lanka antimicrobials for treatment of dogs are available over the counter. Important is the finding of novel type V(T) SCCmec elements, which further underlines the high recombination frequency of SCC elements. The ΨSCCmec57395 was found in isolates of CC45, which is the only sequence type of MRSP known to contain this pseudo-cassette.

KW - S. pseudintermedius

KW - SCCmec variants

KW - Sri Lanka

KW - ΨSCCmec

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DO - 10.1016/j.vetmic.2017.11.022

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JF - Veterinary Microbiology

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