Methanol coneversion by a novel thermophilic homoacetogenic bacterium Moorella mulderi sp.nov. isolated from a bioreactor

M. Balk, J. Weijma, M.W. Friedrich, A.J.M. Stams

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

A thermophilic, anaerobic, spore-forming bacterium (strain TMS) was isolated from a thermophilic bioreactor operated at 65 degreesC with methanol as the energy source. Cells were gram-positive straight rods, 0.4-0.6 mum x 2-8 mum, growing as single cells or in pairs. The temperature range for growth was 40-70 degreesC with an optimum at 65 degreesC. Growth was observed from pH 5.5 to 8.5, and the optimum pH was around 7. The salinity range for growth was 0-45 g NaCl l(-1) with an optimum at 10 g l(-1). The isolate was able to grow on methanol, H2CO2 (80/20%, v/v), formate, lactate, pyruvate, glucose, fructose, cellobiose and pectin. The bacterium reduced thiosulfate to sulfide. The G+C content of the DNA was 53 mol%. Comparison of 16S rRNA genes revealed that strain TMS is related to Moorella glycerini (96%, sequence similarity), Moorella thermoacetica (92%) and Moorella thermoautotrophica (92%). On the basis of physiological and phylogenetic differences, strain TMS is proposed as a new species within the genus Moorella, Moorella mulderi sp. nov. (=DSM 14980, =ATCC BAA-608).
Original languageEnglish
Pages (from-to)315-320
JournalArchives of Microbiology
Volume179
DOIs
Publication statusPublished - 2003

Keywords

  • gradient gel-electrophoresis
  • sulfate-reducing bacterium
  • 16s ribosomal-rna
  • anaerobic reactor
  • degradation
  • clostridium
  • reduction
  • hydrogen
  • growth
  • spores

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