Abstract
A thermophilic, anaerobic, spore-forming bacterium (strain TMS) was isolated from a thermophilic bioreactor operated at 65 degreesC with methanol as the energy source. Cells were gram-positive straight rods, 0.4-0.6 mum x 2-8 mum, growing as single cells or in pairs. The temperature range for growth was 40-70 degreesC with an optimum at 65 degreesC. Growth was observed from pH 5.5 to 8.5, and the optimum pH was around 7. The salinity range for growth was 0-45 g NaCl l(-1) with an optimum at 10 g l(-1). The isolate was able to grow on methanol, H2CO2 (80/20%, v/v), formate, lactate, pyruvate, glucose, fructose, cellobiose and pectin. The bacterium reduced thiosulfate to sulfide. The G+C content of the DNA was 53 mol%. Comparison of 16S rRNA genes revealed that strain TMS is related to Moorella glycerini (96%, sequence similarity), Moorella thermoacetica (92%) and Moorella thermoautotrophica (92%). On the basis of physiological and phylogenetic differences, strain TMS is proposed as a new species within the genus Moorella, Moorella mulderi sp. nov. (=DSM 14980, =ATCC BAA-608).
Original language | English |
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Pages (from-to) | 315-320 |
Journal | Archives of Microbiology |
Volume | 179 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- gradient gel-electrophoresis
- sulfate-reducing bacterium
- 16s ribosomal-rna
- anaerobic reactor
- degradation
- clostridium
- reduction
- hydrogen
- growth
- spores