Metal-induced tolerance in the freshwater microbenthic diatom Gomphonema parvulum

N. Ivorra, C. Barranguet, M. Jonker, M.H.S. Kraak, W. Admiraal

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

The benthic diatom Gomphonema parvulum Kützing is a common species in both clean and metal contaminated rivers. Our aim was to investigate whether metal-induced tolerance could explain the persistance of this taxon under metal polluted conditions. G. parvulum strains were isolated from a Zn- and Cd-contaminated stream and from a relatively clean ("reference") stream. The strains were cultured in synthetic medium as mono-specific biofilms to maintain their specific benthic growth features. Moreover, the strain from the metal polluted stream was cultured in plain and Zn- and Cd-enriched synthetic medium. Short-term (5 h) toxicity experiments with Zn were performed with the strains using pulse amplitude modulated (PAM) fluorometry. Zn lowered significantly the minimal chlorophyll fluorescence (F0) and the photon yield (p) of the exposed strains after 5 h exposure. The actual Zn concentrations that caused a 50% reduction (EC50's) of the p of the strain from the metal polluted stream were significantly higher than those of the isolate from the unpolluted stream. The absence of tolerance to Cu of the "polluted" strain indicated that Zn tolerance resulted from specific induction by chronic exposure to Zn in the field. Observations on field biofilms confirmed a higher tolerance of the G. parvulum population from the polluted stream than of the G. parvulum population from the reference stream. A genetic nature of this metal adaptation was supported by the persistance of the Zn tolerance of the polluted strain 2 years after isolation.
Original languageEnglish
Pages (from-to)147-157
JournalEnvironmental Pollution
Volume116
Issue number1
DOIs
Publication statusPublished - 2002

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Diatoms
Fresh Water
Metals
Biofilms
Fluorometry
Chlorophyll
Photons
Rivers
Population
Fluorescence
Toxicity
Growth

Keywords

  • fresh water
  • water pollution
  • zinc
  • metal tolerance
  • biological indicators
  • algae

Cite this

Ivorra, N., Barranguet, C., Jonker, M., Kraak, M. H. S., & Admiraal, W. (2002). Metal-induced tolerance in the freshwater microbenthic diatom Gomphonema parvulum. Environmental Pollution, 116(1), 147-157. https://doi.org/10.1016/S0269-7491(01)00152-X
Ivorra, N. ; Barranguet, C. ; Jonker, M. ; Kraak, M.H.S. ; Admiraal, W. / Metal-induced tolerance in the freshwater microbenthic diatom Gomphonema parvulum. In: Environmental Pollution. 2002 ; Vol. 116, No. 1. pp. 147-157.
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Ivorra, N, Barranguet, C, Jonker, M, Kraak, MHS & Admiraal, W 2002, 'Metal-induced tolerance in the freshwater microbenthic diatom Gomphonema parvulum' Environmental Pollution, vol. 116, no. 1, pp. 147-157. https://doi.org/10.1016/S0269-7491(01)00152-X

Metal-induced tolerance in the freshwater microbenthic diatom Gomphonema parvulum. / Ivorra, N.; Barranguet, C.; Jonker, M.; Kraak, M.H.S.; Admiraal, W.

In: Environmental Pollution, Vol. 116, No. 1, 2002, p. 147-157.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Metal-induced tolerance in the freshwater microbenthic diatom Gomphonema parvulum

AU - Ivorra, N.

AU - Barranguet, C.

AU - Jonker, M.

AU - Kraak, M.H.S.

AU - Admiraal, W.

PY - 2002

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N2 - The benthic diatom Gomphonema parvulum Kützing is a common species in both clean and metal contaminated rivers. Our aim was to investigate whether metal-induced tolerance could explain the persistance of this taxon under metal polluted conditions. G. parvulum strains were isolated from a Zn- and Cd-contaminated stream and from a relatively clean ("reference") stream. The strains were cultured in synthetic medium as mono-specific biofilms to maintain their specific benthic growth features. Moreover, the strain from the metal polluted stream was cultured in plain and Zn- and Cd-enriched synthetic medium. Short-term (5 h) toxicity experiments with Zn were performed with the strains using pulse amplitude modulated (PAM) fluorometry. Zn lowered significantly the minimal chlorophyll fluorescence (F0) and the photon yield (p) of the exposed strains after 5 h exposure. The actual Zn concentrations that caused a 50% reduction (EC50's) of the p of the strain from the metal polluted stream were significantly higher than those of the isolate from the unpolluted stream. The absence of tolerance to Cu of the "polluted" strain indicated that Zn tolerance resulted from specific induction by chronic exposure to Zn in the field. Observations on field biofilms confirmed a higher tolerance of the G. parvulum population from the polluted stream than of the G. parvulum population from the reference stream. A genetic nature of this metal adaptation was supported by the persistance of the Zn tolerance of the polluted strain 2 years after isolation.

AB - The benthic diatom Gomphonema parvulum Kützing is a common species in both clean and metal contaminated rivers. Our aim was to investigate whether metal-induced tolerance could explain the persistance of this taxon under metal polluted conditions. G. parvulum strains were isolated from a Zn- and Cd-contaminated stream and from a relatively clean ("reference") stream. The strains were cultured in synthetic medium as mono-specific biofilms to maintain their specific benthic growth features. Moreover, the strain from the metal polluted stream was cultured in plain and Zn- and Cd-enriched synthetic medium. Short-term (5 h) toxicity experiments with Zn were performed with the strains using pulse amplitude modulated (PAM) fluorometry. Zn lowered significantly the minimal chlorophyll fluorescence (F0) and the photon yield (p) of the exposed strains after 5 h exposure. The actual Zn concentrations that caused a 50% reduction (EC50's) of the p of the strain from the metal polluted stream were significantly higher than those of the isolate from the unpolluted stream. The absence of tolerance to Cu of the "polluted" strain indicated that Zn tolerance resulted from specific induction by chronic exposure to Zn in the field. Observations on field biofilms confirmed a higher tolerance of the G. parvulum population from the polluted stream than of the G. parvulum population from the reference stream. A genetic nature of this metal adaptation was supported by the persistance of the Zn tolerance of the polluted strain 2 years after isolation.

KW - zoet water

KW - waterverontreiniging

KW - zink

KW - metaaltolerantie

KW - biologische indicatoren

KW - algen

KW - fresh water

KW - water pollution

KW - zinc

KW - metal tolerance

KW - biological indicators

KW - algae

U2 - 10.1016/S0269-7491(01)00152-X

DO - 10.1016/S0269-7491(01)00152-X

M3 - Article

VL - 116

SP - 147

EP - 157

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

IS - 1

ER -