Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses

Mirka Macel*, I.S.G. Visschers, Janny Peters, I.F. Kappers, C.H. de Vos, Nicole M. van Dam

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources of
resistance in crops. Here, we used natural variation in pepper (Capsicum spp.) resistance combined with an untargeted metabolomics
approach to detect secondary metabolites related to thrips (Frankliniella occidentalis) resistance. Using leaf disc choice
assays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thrips
resistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. The
choice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended on
plant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plant
developmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimer
acyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose and
malonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides of
pepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discovering
metabolites that are important in plant – insect interactions.
Keywords Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips
Original languageEnglish
Pages (from-to)490-501
Number of pages12
JournalJournal of Chemical Ecology
Volume45
Issue number5-6
Early online date8 Jun 2019
DOIs
Publication statusPublished - Jun 2019

Fingerprint

Thysanoptera
Thrips (Thripidae)
Capsicum
thrips
Metabolomics
chemical defense
Diterpenes
Chemical potential
metabolomics
sweet peppers
Glycosides
diterpenoids
Dimers
Insects
glycosides
Metabolites
Monomers
flavone
Frankliniella occidentalis
insect

Keywords

  • Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips

Cite this

@article{dbf619d4b66643dca5e11fac91384cc7,
title = "Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses",
abstract = "The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources ofresistance in crops. Here, we used natural variation in pepper (Capsicum spp.) resistance combined with an untargeted metabolomicsapproach to detect secondary metabolites related to thrips (Frankliniella occidentalis) resistance. Using leaf disc choiceassays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thripsresistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. Thechoice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended onplant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plantdevelopmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimeracyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose andmalonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides ofpepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discoveringmetabolites that are important in plant – insect interactions.Keywords Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips",
keywords = "Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips",
author = "Mirka Macel and I.S.G. Visschers and Janny Peters and I.F. Kappers and {de Vos}, C.H. and {van Dam}, {Nicole M.}",
year = "2019",
month = "6",
doi = "10.1007/s10886-019-01074-4",
language = "English",
volume = "45",
pages = "490--501",
journal = "Journal of Chemical Ecology",
issn = "0098-0331",
publisher = "Springer Verlag",
number = "5-6",

}

Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses. / Macel, Mirka; Visschers, I.S.G.; Peters, Janny; Kappers, I.F.; de Vos, C.H.; van Dam, Nicole M.

In: Journal of Chemical Ecology, Vol. 45, No. 5-6, 06.2019, p. 490-501.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses

AU - Macel, Mirka

AU - Visschers, I.S.G.

AU - Peters, Janny

AU - Kappers, I.F.

AU - de Vos, C.H.

AU - van Dam, Nicole M.

PY - 2019/6

Y1 - 2019/6

N2 - The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources ofresistance in crops. Here, we used natural variation in pepper (Capsicum spp.) resistance combined with an untargeted metabolomicsapproach to detect secondary metabolites related to thrips (Frankliniella occidentalis) resistance. Using leaf disc choiceassays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thripsresistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. Thechoice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended onplant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plantdevelopmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimeracyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose andmalonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides ofpepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discoveringmetabolites that are important in plant – insect interactions.Keywords Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips

AB - The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources ofresistance in crops. Here, we used natural variation in pepper (Capsicum spp.) resistance combined with an untargeted metabolomicsapproach to detect secondary metabolites related to thrips (Frankliniella occidentalis) resistance. Using leaf disc choiceassays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thripsresistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. Thechoice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended onplant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plantdevelopmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimeracyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose andmalonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides ofpepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discoveringmetabolites that are important in plant – insect interactions.Keywords Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips

KW - Capsianosides . Frankliniella occidentalis . Liquid chromatography-mass spectrometry . Insects . Solanaceae . Thrips

U2 - 10.1007/s10886-019-01074-4

DO - 10.1007/s10886-019-01074-4

M3 - Article

VL - 45

SP - 490

EP - 501

JO - Journal of Chemical Ecology

JF - Journal of Chemical Ecology

SN - 0098-0331

IS - 5-6

ER -