Abstract
Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.
Original language | English |
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Article number | dmm047746 |
Journal | Disease Models & Mechanisms |
Volume | 14 |
Issue number | 4 |
DOIs | |
Publication status | Published - 27 Apr 2021 |
Keywords
- C. elegans
- Lipidomics
- Metabolism
- Metabolomics