Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation

Marte Molenaars; Bauke Schomakers; Hyung Elfrink; Arwen W. Gao; Martin Vervaart; Mia Pras-Raves; Angela  Luyf; Reuben L. Smith; M.G. Sterken; J.E. Kammenga; Antoine H.C. Van Kampen; Georges  Janssens; Frederic Vaz; Michel van Weeghel; Riekelt H. Houtkooper

doi:10.1242/dmm.047746

Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation

Marte Molenaars, Bauke Schomakers, Hyung Elfrink, Arwen W. Gao, Martin Vervaart, Mia Pras-Raves, Angela Luyf, Reuben L. Smith, M.G. Sterken, J.E. Kammenga, Antoine H.C. Van Kampen, Georges Janssens, Frederic Vaz, Michel van Weeghel^*, Riekelt H. Houtkooper^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

37 Citations (Scopus)

Abstract

Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.

Original language	English
Article number	dmm047746
Journal	Disease Models & Mechanisms
Volume	14
Issue number	4
DOIs	https://doi.org/10.1242/dmm.047746
Publication status	Published - 27 Apr 2021

Keywords

C. elegans
Lipidomics
Metabolism
Metabolomics

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10.1242/dmm.047746Licence: CC BY

https://edepot.wur.nl/544821Licence: CC BY

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Molenaars, M., Schomakers, B., Elfrink, H., Gao, A. W., Vervaart, M., Pras-Raves, M., Luyf, A., Smith, R. L., Sterken, M. G., Kammenga, J. E., Van Kampen, A. H. C., Janssens, G., Vaz, F., van Weeghel, M., & Houtkooper, R. H. (2021). Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation. Disease Models & Mechanisms, 14(4), Article dmm047746. https://doi.org/10.1242/dmm.047746

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title = "Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation",

abstract = "Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.",

keywords = "C. elegans, Lipidomics, Metabolism, Metabolomics",

author = "Marte Molenaars and Bauke Schomakers and Hyung Elfrink and Gao, {Arwen W.} and Martin Vervaart and Mia Pras-Raves and Angela Luyf and Smith, {Reuben L.} and M.G. Sterken and J.E. Kammenga and {Van Kampen}, {Antoine H.C.} and Georges Janssens and Frederic Vaz and {van Weeghel}, Michel and Houtkooper, {Riekelt H.}",

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doi = "10.1242/dmm.047746",

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Molenaars, M, Schomakers, B, Elfrink, H, Gao, AW, Vervaart, M, Pras-Raves, M, Luyf, A, Smith, RL, Sterken, MG , Kammenga, JE, Van Kampen, AHC, Janssens, G, Vaz, F, van Weeghel, M & Houtkooper, RH 2021, 'Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation', Disease Models & Mechanisms, vol. 14, no. 4, dmm047746. https://doi.org/10.1242/dmm.047746

TY - JOUR

T1 - Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation

AU - Molenaars, Marte

AU - Schomakers, Bauke

AU - Elfrink, Hyung

AU - Gao, Arwen W.

AU - Vervaart, Martin

AU - Pras-Raves, Mia

AU - Luyf, Angela

AU - Smith, Reuben L.

AU - Sterken, M.G.

AU - Kammenga, J.E.

AU - Van Kampen, Antoine H.C.

AU - Janssens, Georges

AU - Vaz, Frederic

AU - van Weeghel, Michel

AU - Houtkooper, Riekelt H.

PY - 2021/4/27

Y1 - 2021/4/27

N2 - Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.

AB - Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.

KW - C. elegans

KW - Lipidomics

KW - Metabolism

KW - Metabolomics

U2 - 10.1242/dmm.047746

DO - 10.1242/dmm.047746

M3 - Article

SN - 1754-8411

VL - 14

JO - Disease Models & Mechanisms

JF - Disease Models & Mechanisms

IS - 4

M1 - dmm047746

ER -

Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation

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