Metabolomics and lipidomics in C. elegans using a single sample preparation

Marte Molenaars, Bauke Schomakers, Hyung Elfrink, Arwen W. Gao, Martin Vervaart, Mia Pras-Raves, Angela Luyf, Reuben L. Smith, M.G. Sterken, J.E. Kammenga, Antoine H.C. Van Kampen, Georges Janssens, Frederic Vaz, Michel van Weeghel, Riekelt H. Houtkooper

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand, for instance in research related to nutrition and aging. The nematode C. elegans is a key model organism in these fields, due to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for instance, glycolysis and the TCA cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.
Original languageEnglish
JournalDisease Models & Mechanisms
DOIs
Publication statusAccepted/In press - 2 Mar 2021

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