Metabolomics analysis of postharvest ripening heterogeneity of ‘Hass' avocadoes

R. Pedreschi, P. Munoz, P. Robledo, C. Becerra, B.G. Defilippi, H.D.L.M. van Eekelen, R. Mumm, E.H. Westra, R.C.H. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)

Abstract

The complex physiology of ‘Hass’ avocado renders its postharvest ripening heterogeneous and unpre-dictable. Several approaches have previously been undertaken to broaden our understanding of the causesof this postharvest ripening heterogeneity but without much success. In this study, a fruit biopsy method-ology was undertaken to sample mesocarp tissue from a series of individual avocado fruit while followingindividual fruit postharvest ripening characteristics without significantly disturbing their metabolism.Using both targeted and untargeted metabolomics approaches, we analyzed the metabolite profiles of thebiopsies in order to get more insight into the biochemical mechanisms underlying ‘Hass’ avocado ripen-ing heterogeneity. While C7sugars (mannoheptulose and perseitol), dry matter and total Ca2+were notcorrelated with time to reach edible ripeness, untargeted metabolomics profiling of polar and semi-polarcompounds (based on GC–MS and LC–MS platforms), revealed several metabolites, mainly amino acids,that were related to ripening heterogeneity. In addition, analysis of fatty acids revealed linoleic acid tobe differentially accumulating. In general, slowest ripening avocados had lower amounts of precursors ofmetabolites involved in key metabolic pathways. Our study indicates that comprehensive metabolomicsmay provide new markers for avocado ripening stage at harvest, and may give more insight into thecomplex ripening physiology of this fruit.
Original languageEnglish
Pages (from-to)172-179
JournalPostharvest Biology and Technology
Volume92
DOIs
Publication statusPublished - 2014

Fingerprint

Persea
Metabolomics
avocados
metabolomics
ripening
Fruit
fruits
Mannoheptulose
physiology
Linoleic Acid
Metabolic Networks and Pathways
metabolites
Gas Chromatography-Mass Spectrometry
biochemical mechanisms
mesocarp
Fatty Acids
Biopsy
Amino Acids
biochemical pathways
linoleic acid

Keywords

  • mass-spectrometry
  • persea-americana
  • fatty-acid
  • low-oxygen
  • fruit
  • metabolite
  • quality
  • sugars
  • maturity
  • ethylene

Cite this

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title = "Metabolomics analysis of postharvest ripening heterogeneity of ‘Hass' avocadoes",
abstract = "The complex physiology of ‘Hass’ avocado renders its postharvest ripening heterogeneous and unpre-dictable. Several approaches have previously been undertaken to broaden our understanding of the causesof this postharvest ripening heterogeneity but without much success. In this study, a fruit biopsy method-ology was undertaken to sample mesocarp tissue from a series of individual avocado fruit while followingindividual fruit postharvest ripening characteristics without significantly disturbing their metabolism.Using both targeted and untargeted metabolomics approaches, we analyzed the metabolite profiles of thebiopsies in order to get more insight into the biochemical mechanisms underlying ‘Hass’ avocado ripen-ing heterogeneity. While C7sugars (mannoheptulose and perseitol), dry matter and total Ca2+were notcorrelated with time to reach edible ripeness, untargeted metabolomics profiling of polar and semi-polarcompounds (based on GC–MS and LC–MS platforms), revealed several metabolites, mainly amino acids,that were related to ripening heterogeneity. In addition, analysis of fatty acids revealed linoleic acid tobe differentially accumulating. In general, slowest ripening avocados had lower amounts of precursors ofmetabolites involved in key metabolic pathways. Our study indicates that comprehensive metabolomicsmay provide new markers for avocado ripening stage at harvest, and may give more insight into thecomplex ripening physiology of this fruit.",
keywords = "mass-spectrometry, persea-americana, fatty-acid, low-oxygen, fruit, metabolite, quality, sugars, maturity, ethylene",
author = "R. Pedreschi and P. Munoz and P. Robledo and C. Becerra and B.G. Defilippi and {van Eekelen}, H.D.L.M. and R. Mumm and E.H. Westra and {de Vos}, R.C.H.",
year = "2014",
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language = "English",
volume = "92",
pages = "172--179",
journal = "Postharvest Biology and Technology",
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}

Metabolomics analysis of postharvest ripening heterogeneity of ‘Hass' avocadoes. / Pedreschi, R.; Munoz, P.; Robledo, P.; Becerra, C.; Defilippi, B.G.; van Eekelen, H.D.L.M.; Mumm, R.; Westra, E.H.; de Vos, R.C.H.

In: Postharvest Biology and Technology, Vol. 92, 2014, p. 172-179.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Pedreschi, R.

AU - Munoz, P.

AU - Robledo, P.

AU - Becerra, C.

AU - Defilippi, B.G.

AU - van Eekelen, H.D.L.M.

AU - Mumm, R.

AU - Westra, E.H.

AU - de Vos, R.C.H.

PY - 2014

Y1 - 2014

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AB - The complex physiology of ‘Hass’ avocado renders its postharvest ripening heterogeneous and unpre-dictable. Several approaches have previously been undertaken to broaden our understanding of the causesof this postharvest ripening heterogeneity but without much success. In this study, a fruit biopsy method-ology was undertaken to sample mesocarp tissue from a series of individual avocado fruit while followingindividual fruit postharvest ripening characteristics without significantly disturbing their metabolism.Using both targeted and untargeted metabolomics approaches, we analyzed the metabolite profiles of thebiopsies in order to get more insight into the biochemical mechanisms underlying ‘Hass’ avocado ripen-ing heterogeneity. While C7sugars (mannoheptulose and perseitol), dry matter and total Ca2+were notcorrelated with time to reach edible ripeness, untargeted metabolomics profiling of polar and semi-polarcompounds (based on GC–MS and LC–MS platforms), revealed several metabolites, mainly amino acids,that were related to ripening heterogeneity. In addition, analysis of fatty acids revealed linoleic acid tobe differentially accumulating. In general, slowest ripening avocados had lower amounts of precursors ofmetabolites involved in key metabolic pathways. Our study indicates that comprehensive metabolomicsmay provide new markers for avocado ripening stage at harvest, and may give more insight into thecomplex ripening physiology of this fruit.

KW - mass-spectrometry

KW - persea-americana

KW - fatty-acid

KW - low-oxygen

KW - fruit

KW - metabolite

KW - quality

KW - sugars

KW - maturity

KW - ethylene

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DO - 10.1016/j.postharvbio.2014.01.024

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JO - Postharvest Biology and Technology

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SN - 0925-5214

ER -