Metabolic shifts: a fitness perspective for microbial cell factories

A. Goel, M.T. Wortel, D. Molenaar, B. Teusink

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

Performance of industrial microorganisms as cell factories is limited by the capacity to channel nutrients to desired products, of which optimal production usually requires careful manipulation of process conditions, or strain improvement. The focus in process improvement is often on understanding and manipulating the regulation of metabolism. Nonetheless, one encounters situations where organisms are remarkably resilient to further optimization or their properties become unstable. Therefore it is important to understand the origin of these apparent limitations to find whether and how they can be improved. We argue that by considering fitness effects of regulation, a more generic explanation for certain behaviour can be obtained. In this view, apparent process limitations arise from trade-offs that cells faced as they evolved to improve fitness. A deeper understanding of such trade-offs using a systems biology approach can ultimately enhance performance of cell factories.
Original languageEnglish
Pages (from-to)2147-2160
JournalBiotechnology Letters
Volume34
Issue number12
DOIs
Publication statusPublished - 2012

Keywords

  • lactic-acid bacteria
  • heterologous protein secretion
  • escherichia-coli
  • saccharomyces-cerevisiae
  • lactococcus-lactis
  • bacillus-subtilis
  • pyruvate metabolism
  • overflow metabolism
  • aerobic glycolysis
  • glucose-metabolism

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