Metabolic response of Alicycliphilus denitrificans strain BC towards electron acceptor variation

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Abstract

Alicycliphilus denitrificans is a versatile, ubiquitous, facultative anaerobic bacterium. A. denitrificans strain BC can use chlorate, nitrate and oxygen as electron acceptor for growth. Cells display a prolonged lag-phase when transferred from nitrate to chlorate and vice versa. Furthermore, cells adapted to aerobic growth do not easily use nitrate or chlorate as electron acceptor. We further investigated these responses of strain BC by differential proteomics, transcript analysis and enzyme activity assays. In nitrate-adapted cells transferred to chlorate and vice versa, appropriate electron acceptor reduction pathways need to be activated. In oxygen-adapted cells, adaptation to the use of chlorate or nitrate is likely difficult due to the poorly active nitrate reduction pathway and low active chlorate reduction pathway. We deduce that the Nar-type nitrate reductase of strain BC also reduces chlorate, which may result in toxic levels of chlorite if cells are transferred to chlorate. Furthermore, the activities of nitrate reductase and nitrite reductase appear to be not balanced when oxygen-adapted cells a shifted to nitrate as electron acceptor, leading to the production of a toxic amount of nitrite. These data suggest that strain BC encounters metabolic challenges in environments with fluctuations in the availability of electron acceptors
Original languageEnglish
Pages (from-to)2886-2894
JournalProteomics
Volume13
Issue number18-19
DOIs
Publication statusPublished - 2013

Keywords

  • microbial perchlorate reduction
  • pseudomonas-chloritidismutans
  • phylogenetic analysis
  • nitrate reductases
  • chlorite dismutase
  • reducing bacteria
  • anaerobic growth
  • chlorate
  • genes
  • identification

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