Metabolic modelling and energy parameter estimation of Tetradesmus obliquus

G.M. León-Saiki, Narcís Ferrer Ledo, David Lao-Martil, Douwe van der Veen, René H. Wijffels, Dirk E. Martens*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

We developed a metabolic network describing the primary metabolism of Tetradesmus obliquus aimed to get a better understanding of metabolism to improve industrial production. The network includes 351 reactions with 183 metabolites distributed over 4 compartments: cytosol, chloroplast, mitochondria, and extracellular space. The energy requirements for biomass assembly and maintenance (Kx and mATP, respectively) were experimentally determined from batch cultures and included in the model. The determined values were 121.02 mmolATP·gDW −1 for Kx and 0.66 mmolATP·gDW −1·h−1 for the mATP. The maintenance value found for T. obliquus is, to our knowledge, one of the lowest reported in literature for microalgae. This low value is also in agreement with the photon maintenance requirement found experimentally for T. obliquus (1.18 mmolph·gDW −1·h−1). Finally, the theoretical maximum yields based on the model for biomass, triacylglycerides (TAG), and starch yield on light were calculated to be 1.15 g·molph −1, 1.05 gTAG·molph −1, and 2.69 gstarch·molph −1.

Original languageEnglish
Pages (from-to)378-387
JournalAlgal Research
Volume35
DOIs
Publication statusPublished - 1 Nov 2018

Keywords

  • Compartmentalized metabolism
  • Flux Balance Analysis (FBA)
  • Maintenance requirement
  • Microalgae
  • Scenedesmus obliquus

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