Metabolic Engineering of TCA Cycle for Production of Chemicals

K.S. Vuoristo, A.E. Mars, J.P.M. Sanders, G. Eggink, R.A. Weusthuis

Research output: Contribution to journalArticleAcademicpeer-review

68 Citations (Scopus)


The tricarboxylic acid (TCA) cycle has been used for decades in the microbial production of chemicals such as citrate, L-glutamate, and succinate. Maximizing yield is key for cost-competitive production. However, for most TCA cycle products, the maximum pathway yield is lower than the theoretical maximum yield (YE). For succinate, this was solved by creating two pathways to the product, using both branches of the TCA cycle, connected by the glyoxylate shunt (GS). A similar solution cannot be applied directly for production of compounds from the oxidative branch of the TCA cycle because irreversible reactions are involved. Here, we describe how this can be overcome and what the impact is on the yield.

Metabolic Engineering of TCA Cycle for Production of Chemicals (PDF Download Available). Available from: [accessed Feb 5, 2016].
Original languageEnglish
Pages (from-to)191-197
JournalTrends in Biotechnology
Issue number3
Publication statusPublished - 2016


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