Metabolic Engineering of Mannitol Production in Lactococcus lactis: Influence of Overexpression of Mannitol 1-Phosphate Dehydrogenase in Different Genetic Backgrounds

H.W. Wisselink, A.E. Mars, P. van der Meer, G. Eggink, J. Hugenholtz

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

To obtain a mannitol-producing Lactococcus lactis strain, the mannitol 1-phosphate dehydrogenase gene (mtlD) from Lactobacillus plantarum was overexpressed in a wild-type strain, a lactate dehydrogenase(LDH)-deficient strain, and a strain with reduced phosphofructokinase activity. High-performance liquid chromatography and 13C nuclear magnetic resonance analysis revealed that small amounts (
Original languageEnglish
Pages (from-to)4286-4292
JournalApplied and Environmental Microbiology
Volume70
Issue number7
DOIs
Publication statusPublished - 2004

Keywords

  • complete genome sequence
  • lactate-dehydrogenase
  • lactobacillus-plantarum
  • acid bacteria
  • phosphotransferase system
  • expression systems
  • protects
  • nisin
  • phosphorylation
  • activation

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