Metabolic engineering of lactic acid bacteria for the production of nutraceuticals

J. Hugenholtz, W. Sybesma, M.N. Groot, W. Wisselink, V. Ladero, K. Burgess, D. van Sinderen, J.C. Piard, G. Eggink, E.J. Smid, G. Savoy, F. Sesma, T. Jansen, P. Hols, M. Kleerebezem

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142 Citations (Scopus)


Lactic acid bacteria display a relatively simple and well-described metabolism where the sugar source is converted mainly to lactic acid. Here we will shortly describe metabolic engineering strategies on the level of sugar metabolism, that lead to either the efficient re-routing of the lactococcal sugar metabolism to nutritional end-products other than lactic acid such as L-alanine, several low-calorie sugars and oligosaccharides or to enhancement of sugar metabolism for complete removal of (undesirable) sugars from food materials. Moreover, we will review current metabolic engineering approaches that aim at increasing the flux through complex biosynthetic pathways, leading to the production of the B-vitamins folate and riboflavin. An overview of these metabolic engineering activities can be found on the website of the Nutra Cells 5th Framework EU-project ( Finally, the impact of the developments in the area of genomics and corresponding high-throughput technologies on nutraceutical production will be discussed
Original languageEnglish
Pages (from-to)217-235
JournalAntonie van Leeuwenhoek: Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie
Issue number1-4
Publication statusPublished - 2002


  • controlled gene-expression
  • lactococcus-lactis
  • exopolysaccharide biosynthesis
  • streptococcus-thermophilus
  • lactobacillus-plantarum
  • glycosyltransferase genes
  • nucleotide-sequence
  • diacetyl production
  • functional-analysis
  • nmr-spectroscopy


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