Metabolic engineering of itaconate production in Escherichia coli

K.S. Vuoristo, A.E. Mars, J. Vidal Sangra, J. Springer, G. Eggink, J.P.M. Sanders, R.A. Weusthuis

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)


Interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. Itaconic acid, a C5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (CadA) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into itaconate. Heterologous expression of cadA from Aspergillus terreus in Escherichia coli resulted in low CadA activities and production of trace amounts of itaconate on Luria-Bertani (LB) medium (
Original languageEnglish
Pages (from-to)221-228
JournalApplied Microbiology and Biotechnology
Issue number1
Publication statusPublished - 2015


  • aconitic acid decarboxylase
  • aspergillus-terreus
  • biotechnological production
  • acetate accumulation
  • citrate synthase
  • lactic-acid
  • fermentation
  • growth
  • expression
  • mutant


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