Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics

    Research output: Contribution to journalArticleAcademicpeer-review

    103 Citations (Scopus)

    Abstract

    Flavonoids comprise a large and diverse group of polyphenolic plant secondary metabolites. In plants, flavonoids play important roles in many biological processes such as pigmentation of flowers, fruits and vegetables, plant-pathogen interactions, fertility and protection against UV light. Being natural plant compounds, flavonoids are an integral part of the human diet and there is increasing evidence that dietary polyphenols are likely candidates for the observed beneficial effects of a diet rich in fruits and vegetables on the prevention of several chronic diseases. Within the plant kingdom, and even within a single plant species, there is a large variation in the levels and composition of flavonoids. This variation is often due to specific mutations in flavonoid-related genes leading to quantitative and qualitative differences in metabolic profiles. The use of such specific flavonoid mutants with easily scorable, visible phenotypes has led to the isolation and characterisation of many structural and regulatory genes involved in the flavonoid biosynthetic pathway from different plant species. These genes have been used to engineer the flavonoid biosynthetic pathway in both model and crop plant species, not only from a fundamental perspective, but also in order to alter important agronomic traits, such as flower and fruit colour, resistance, nutritional value. This review describes the advances made in engineering the flavonoid pathway in tomato (Solanum lycopersicum). Three different approaches will be described; (I) Increasing endogenous tomato flavonoids using structural or regulatory genes; (II) Blocking specific steps in the flavonoid pathway by RNA interference strategies; and (III) Production of novel tomato flavonoids by introducing novel branches of the flavonoid pathway. Metabolite profiling is an essential tool to analyse the effects of pathway engineering approaches, not only to analyse the effect on the flavonoid composition itself, but also on other related or unrelated metabolic pathways. Metabolomics will therefore play an increasingly important role in revealing a more complete picture of metabolic perturbation and will provide additional novel insights into the effect of the introduced genes and the role of flavonoids in plant physiology and development.
    Original languageEnglish
    Pages (from-to)399-412
    JournalMetabolomics
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Metabolic engineering
    Metabolic Engineering
    Metabolomics
    Lycopersicon esculentum
    Flavonoids
    Genes
    Fruits
    Fruit
    Biosynthetic Pathways
    Vegetables
    Regulator Genes
    Nutrition
    Metabolites
    Plant Physiological Phenomena
    Diet
    Biological Phenomena
    Plant Development
    Metabolome
    Nutritive Value
    Physiology

    Keywords

    • signal-transduction pathways
    • flight mass-spectrometry
    • stilbene synthase gene
    • anthocyanin biosynthesis
    • flower color
    • chalcone synthase
    • phenylpropanoid metabolism
    • heterologous expression
    • antioxidant activity
    • divergent evolution

    Cite this

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    title = "Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics",
    abstract = "Flavonoids comprise a large and diverse group of polyphenolic plant secondary metabolites. In plants, flavonoids play important roles in many biological processes such as pigmentation of flowers, fruits and vegetables, plant-pathogen interactions, fertility and protection against UV light. Being natural plant compounds, flavonoids are an integral part of the human diet and there is increasing evidence that dietary polyphenols are likely candidates for the observed beneficial effects of a diet rich in fruits and vegetables on the prevention of several chronic diseases. Within the plant kingdom, and even within a single plant species, there is a large variation in the levels and composition of flavonoids. This variation is often due to specific mutations in flavonoid-related genes leading to quantitative and qualitative differences in metabolic profiles. The use of such specific flavonoid mutants with easily scorable, visible phenotypes has led to the isolation and characterisation of many structural and regulatory genes involved in the flavonoid biosynthetic pathway from different plant species. These genes have been used to engineer the flavonoid biosynthetic pathway in both model and crop plant species, not only from a fundamental perspective, but also in order to alter important agronomic traits, such as flower and fruit colour, resistance, nutritional value. This review describes the advances made in engineering the flavonoid pathway in tomato (Solanum lycopersicum). Three different approaches will be described; (I) Increasing endogenous tomato flavonoids using structural or regulatory genes; (II) Blocking specific steps in the flavonoid pathway by RNA interference strategies; and (III) Production of novel tomato flavonoids by introducing novel branches of the flavonoid pathway. Metabolite profiling is an essential tool to analyse the effects of pathway engineering approaches, not only to analyse the effect on the flavonoid composition itself, but also on other related or unrelated metabolic pathways. Metabolomics will therefore play an increasingly important role in revealing a more complete picture of metabolic perturbation and will provide additional novel insights into the effect of the introduced genes and the role of flavonoids in plant physiology and development.",
    keywords = "signal-transduction pathways, flight mass-spectrometry, stilbene synthase gene, anthocyanin biosynthesis, flower color, chalcone synthase, phenylpropanoid metabolism, heterologous expression, antioxidant activity, divergent evolution",
    author = "A.G. Bovy and E.G.W.M. Schijlen and R.D. Hall",
    year = "2007",
    doi = "10.1007/s11306-007-0074-2",
    language = "English",
    volume = "3",
    pages = "399--412",
    journal = "Metabolomics",
    issn = "1573-3882",
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    }

    Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics. / Bovy, A.G.; Schijlen, E.G.W.M.; Hall, R.D.

    In: Metabolomics, Vol. 3, No. 3, 2007, p. 399-412.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics

    AU - Bovy, A.G.

    AU - Schijlen, E.G.W.M.

    AU - Hall, R.D.

    PY - 2007

    Y1 - 2007

    N2 - Flavonoids comprise a large and diverse group of polyphenolic plant secondary metabolites. In plants, flavonoids play important roles in many biological processes such as pigmentation of flowers, fruits and vegetables, plant-pathogen interactions, fertility and protection against UV light. Being natural plant compounds, flavonoids are an integral part of the human diet and there is increasing evidence that dietary polyphenols are likely candidates for the observed beneficial effects of a diet rich in fruits and vegetables on the prevention of several chronic diseases. Within the plant kingdom, and even within a single plant species, there is a large variation in the levels and composition of flavonoids. This variation is often due to specific mutations in flavonoid-related genes leading to quantitative and qualitative differences in metabolic profiles. The use of such specific flavonoid mutants with easily scorable, visible phenotypes has led to the isolation and characterisation of many structural and regulatory genes involved in the flavonoid biosynthetic pathway from different plant species. These genes have been used to engineer the flavonoid biosynthetic pathway in both model and crop plant species, not only from a fundamental perspective, but also in order to alter important agronomic traits, such as flower and fruit colour, resistance, nutritional value. This review describes the advances made in engineering the flavonoid pathway in tomato (Solanum lycopersicum). Three different approaches will be described; (I) Increasing endogenous tomato flavonoids using structural or regulatory genes; (II) Blocking specific steps in the flavonoid pathway by RNA interference strategies; and (III) Production of novel tomato flavonoids by introducing novel branches of the flavonoid pathway. Metabolite profiling is an essential tool to analyse the effects of pathway engineering approaches, not only to analyse the effect on the flavonoid composition itself, but also on other related or unrelated metabolic pathways. Metabolomics will therefore play an increasingly important role in revealing a more complete picture of metabolic perturbation and will provide additional novel insights into the effect of the introduced genes and the role of flavonoids in plant physiology and development.

    AB - Flavonoids comprise a large and diverse group of polyphenolic plant secondary metabolites. In plants, flavonoids play important roles in many biological processes such as pigmentation of flowers, fruits and vegetables, plant-pathogen interactions, fertility and protection against UV light. Being natural plant compounds, flavonoids are an integral part of the human diet and there is increasing evidence that dietary polyphenols are likely candidates for the observed beneficial effects of a diet rich in fruits and vegetables on the prevention of several chronic diseases. Within the plant kingdom, and even within a single plant species, there is a large variation in the levels and composition of flavonoids. This variation is often due to specific mutations in flavonoid-related genes leading to quantitative and qualitative differences in metabolic profiles. The use of such specific flavonoid mutants with easily scorable, visible phenotypes has led to the isolation and characterisation of many structural and regulatory genes involved in the flavonoid biosynthetic pathway from different plant species. These genes have been used to engineer the flavonoid biosynthetic pathway in both model and crop plant species, not only from a fundamental perspective, but also in order to alter important agronomic traits, such as flower and fruit colour, resistance, nutritional value. This review describes the advances made in engineering the flavonoid pathway in tomato (Solanum lycopersicum). Three different approaches will be described; (I) Increasing endogenous tomato flavonoids using structural or regulatory genes; (II) Blocking specific steps in the flavonoid pathway by RNA interference strategies; and (III) Production of novel tomato flavonoids by introducing novel branches of the flavonoid pathway. Metabolite profiling is an essential tool to analyse the effects of pathway engineering approaches, not only to analyse the effect on the flavonoid composition itself, but also on other related or unrelated metabolic pathways. Metabolomics will therefore play an increasingly important role in revealing a more complete picture of metabolic perturbation and will provide additional novel insights into the effect of the introduced genes and the role of flavonoids in plant physiology and development.

    KW - signal-transduction pathways

    KW - flight mass-spectrometry

    KW - stilbene synthase gene

    KW - anthocyanin biosynthesis

    KW - flower color

    KW - chalcone synthase

    KW - phenylpropanoid metabolism

    KW - heterologous expression

    KW - antioxidant activity

    KW - divergent evolution

    U2 - 10.1007/s11306-007-0074-2

    DO - 10.1007/s11306-007-0074-2

    M3 - Article

    VL - 3

    SP - 399

    EP - 412

    JO - Metabolomics

    JF - Metabolomics

    SN - 1573-3882

    IS - 3

    ER -