Metabolic diversity in apple germplasm

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

We analysed metabolic diversity in apples from wild species, elite material and a F1 population, using liquid chromatography–mass spectrometry (LC-QTOF-MS). The evaluated elite material appeared to have strongly reduced levels of phenolic compounds, down to 1% of the concentrations in the investigated wild germplasm. In one quarter of the F1 population, the concentrations of phenolic compounds such as quercetin derivatives, procyanidin, catechin and epicatechin were further significantly reduced, due to accumulation of recessive alleles of putatively leucoanthocyanidin reductase, a structural gene that is located at the top of LG16. In another part of F1 progeny, putatively glycosylated forms of ß-glycols were up to 50 times more abundant compared to both parents. These metabolites were mapped with high logarithm of odds (LOD) scores at the top of LG8, and progeny that was homozygous recessive for the candidate gene showed the elevated levels. We hypothesize that this was caused by inheritance of non-functional alleles of enoyl-CoA hydratase gene. Both examples of
LanguageEnglish
Pages281-290
JournalPlant Breeding
Volume133
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Catechin
Malus
phenolic compounds
germplasm
Enoyl-CoA Hydratase
apples
Alleles
alleles
Recessive Genes
glycols
Glycols
Anthocyanins
structural genes
recessive genes
Quercetin
epicatechin
catechin
Liquid Chromatography
quercetin
liquid chromatography

Keywords

  • x domestica borkh.
  • linkage group 16
  • phenolic-compounds
  • genetic diversity
  • malus-sieversii
  • mqtl hotspot
  • rosaceae
  • origin
  • fruits
  • genome

Cite this

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title = "Metabolic diversity in apple germplasm",
abstract = "We analysed metabolic diversity in apples from wild species, elite material and a F1 population, using liquid chromatography–mass spectrometry (LC-QTOF-MS). The evaluated elite material appeared to have strongly reduced levels of phenolic compounds, down to 1{\%} of the concentrations in the investigated wild germplasm. In one quarter of the F1 population, the concentrations of phenolic compounds such as quercetin derivatives, procyanidin, catechin and epicatechin were further significantly reduced, due to accumulation of recessive alleles of putatively leucoanthocyanidin reductase, a structural gene that is located at the top of LG16. In another part of F1 progeny, putatively glycosylated forms of {\ss}-glycols were up to 50 times more abundant compared to both parents. These metabolites were mapped with high logarithm of odds (LOD) scores at the top of LG8, and progeny that was homozygous recessive for the candidate gene showed the elevated levels. We hypothesize that this was caused by inheritance of non-functional alleles of enoyl-CoA hydratase gene. Both examples of",
keywords = "x domestica borkh., linkage group 16, phenolic-compounds, genetic diversity, malus-sieversii, mqtl hotspot, rosaceae, origin, fruits, genome",
author = "S.A. Khan and Y.M. Tikunov and P.Y.F.R.P. Chibon and C.A. Maliepaard and M.J. Beekwilder and E. Jacobsen and H.J. Schouten",
year = "2014",
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journal = "Plant Breeding",
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Metabolic diversity in apple germplasm. / Khan, S.A.; Tikunov, Y.M.; Chibon, P.Y.F.R.P.; Maliepaard, C.A.; Beekwilder, M.J.; Jacobsen, E.; Schouten, H.J.

In: Plant Breeding, Vol. 133, No. 2, 2014, p. 281-290.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Metabolic diversity in apple germplasm

AU - Khan, S.A.

AU - Tikunov, Y.M.

AU - Chibon, P.Y.F.R.P.

AU - Maliepaard, C.A.

AU - Beekwilder, M.J.

AU - Jacobsen, E.

AU - Schouten, H.J.

PY - 2014

Y1 - 2014

N2 - We analysed metabolic diversity in apples from wild species, elite material and a F1 population, using liquid chromatography–mass spectrometry (LC-QTOF-MS). The evaluated elite material appeared to have strongly reduced levels of phenolic compounds, down to 1% of the concentrations in the investigated wild germplasm. In one quarter of the F1 population, the concentrations of phenolic compounds such as quercetin derivatives, procyanidin, catechin and epicatechin were further significantly reduced, due to accumulation of recessive alleles of putatively leucoanthocyanidin reductase, a structural gene that is located at the top of LG16. In another part of F1 progeny, putatively glycosylated forms of ß-glycols were up to 50 times more abundant compared to both parents. These metabolites were mapped with high logarithm of odds (LOD) scores at the top of LG8, and progeny that was homozygous recessive for the candidate gene showed the elevated levels. We hypothesize that this was caused by inheritance of non-functional alleles of enoyl-CoA hydratase gene. Both examples of

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KW - linkage group 16

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KW - genetic diversity

KW - malus-sieversii

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KW - rosaceae

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KW - fruits

KW - genome

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