Metabolic and transcriptomic changes induced in Arabidopsis by the rhizobacterium Pseudomonas fluorescens SS101

J.E. van de Mortel, R.C.H. de Vos, E. Dekkers, A. Pineda, L. Guillod, K. Bouwmeester, J.J.A. van Loon, M. Dicke, J.M. Raaijmakers

Research output: Contribution to journalArticleAcademicpeer-review

141 Citations (Scopus)

Abstract

Systemic resistance induced in plants by nonpathogenic rhizobacteria is typically effective against multiple pathogens. Here, we show that root-colonizing Pseudomonas fluorescens strain SS101 (Pf.SS101) enhanced resistance in Arabidopsis (Arabidopsis thaliana) against several bacterial pathogens, including Pseudomonas syringae pv tomato (Pst) and the insect pest Spodoptera exigua. Transcriptomic analysis and bioassays with specific Arabidopsis mutants revealed that, unlike many other rhizobacteria, the Pf.SS101-induced resistance response to Pst is dependent on salicylic acid signaling and not on jasmonic acid and ethylene signaling. Genome-wide transcriptomic and untargeted metabolomic analyses showed that in roots and leaves of Arabidopsis plants treated with Pf.SS101, approximately 1,910 genes and 50 metabolites were differentially regulated relative to untreated plants. Integration of both sets of “omics” data pointed to a prominent role of camalexin and glucosinolates in the Pf.SS101-induced resistance response. Subsequent bioassays with seven Arabidopsis mutants (myb51, cyp79B2cyp79B3, cyp81F2, pen2, cyp71A12, cyp71A13, and myb28myb29) disrupted in the biosynthesis pathways for these plant secondary metabolites showed that camalexin and glucosinolates are indeed required for the induction of Pst resistance by Pf.SS101. Also for the insect S. exigua, the indolic glucosinolates appeared to play a role in the Pf.SS101-induced resistance response. This study provides, to our knowledge for the first time, insight into the substantial biochemical and temporal transcriptional changes in Arabidopsis associated with the salicylic acid-dependent resistance response induced by specific rhizobacteria.
Original languageEnglish
Pages (from-to)2173-2188
JournalPlant Physiology
Volume160
Issue number4
DOIs
Publication statusPublished - 2012

Keywords

  • induced systemic resistance
  • growth-promoting rhizobacteria
  • tobacco necrosis virus
  • syringae pv. tomato
  • salicylic-acid
  • acquired-resistance
  • aeruginosa 7nsk2
  • gene-expression
  • beneficial microbes
  • disease resistance

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