A study was conducted to investigate the metabolic activity in germinating transgenic tomato cv. Money Maker seeds not related to distribution of free water within the seed. The parameters measured were in situ luciferase activity, proton distribution density and ATP distribution during germination. Luciferase activity was mainly observed in the endosperm cap region throughout the germination process. The highest proton density (resembling free water) is visible in the lateral endosperm. ATP was observed in the embryo and endosperm throughout the germination process. Results showed that metabolic activity as measured by luciferase activity and ATP accumulation is independent of the distribution of proton density, predominantly resembling free water.
|Title of host publication||The biology of seeds:|
|Subtitle of host publication||recent research advances. Proceedings of the Seventh Internation al Workshop on Seeds, Salamanca, Spain, 2002|
|Editors||G. Nicolás, K.J. Bradford, D. Côme, H.W. Pritchard|
|Publication status||Published - 2003|