Meta-analysis of six dairy cattle breeds reveals biologically relevant candidate genes for mastitis resistance

Zexi Cai*, Terhi Iso-Touru, Marie Pierre Sanchez, Naveen Kadri, Aniek C. Bouwman, Praveen Krishna Chitneedi, Iona M. MacLeod, Christy J. Vander Jagt, Amanda J. Chamberlain, Birgit Gredler-Grandl, Mirjam Spengeler, Mogens Sandø Lund, Didier Boichard, Christa Kühn, Hubert Pausch, Johanna Vilkki, Goutam Sahana

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Mastitis is a disease that incurs significant costs in the dairy industry. A promising approach to mitigate its negative effects is to genetically improve the resistance of dairy cattle to mastitis. A meta-analysis of genome-wide association studies (GWAS) across multiple breeds for clinical mastitis (CM) and its indicator trait, somatic cell score (SCS), is a powerful method to identify functional genetic variants that impact mastitis resistance. Results: We conducted meta-analyses of eight and fourteen GWAS on CM and SCS, respectively, using 30,689 and 119,438 animals from six dairy cattle breeds. Methods for the meta-analyses were selected to properly account for the multi-breed structure of the GWAS data. Our study revealed 58 lead markers that were associated with mastitis incidence, including 16 loci that did not overlap with previously identified quantitative trait loci (QTL), as curated at the Animal QTLdb. Post-GWAS analysis techniques such as gene-based analysis and genomic feature enrichment analysis enabled prioritization of 31 candidate genes and 14 credible candidate causal variants that affect mastitis. Conclusions: Our list of candidate genes can help to elucidate the genetic architecture underlying mastitis resistance and provide better tools for the prevention or treatment of mastitis, ultimately contributing to more sustainable animal production.

Original languageEnglish
Article number54
Number of pages22
JournalGenetics Selection Evolution
Volume56
DOIs
Publication statusPublished - 15 Jul 2024

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