Meiotic recombination profiling of interspecific hybrid F1 tomato pollen by linked read sequencing

Roven Fuentes, T. Hesselink, Ronald Nieuwenhuis, L.V. Bakker, E.G.W.M. Schijlen, W. van Dooijeweert, S. Diaz Trivino, Jorn R. de Haan, G.F. Sanchez Perez, Xinyue Zhang, Paul Fransz, J.H.S.G.M. de Jong, A.D.J. van Dijk, D. de Ridder, S.A. Peters*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Genome wide screening of pooled pollen samples from a single interspecific F1 hybrid obtained from a cross between tomato, Solanum lycopersicum and its wild relative, Solanum pimpinellifolium using linked read sequencing of the haploid nuclei, allowed profiling of the crossover (CO) and gene conversion (GC) landscape. We observed a striking overlap between cold regions of CO in the male gametes and our previously established F6 recombinant inbred lines (RILs) population. COs were overrepresented in non‐coding regions in the gene promoter and 5′UTR regions of genes. Poly‐A/T and AT rich motifs were found enriched in 1 kb promoter regions flanking the CO sites. Non‐crossover associated allelic and ectopic GCs were detected in most chromosomes, confirming that besides CO, GC represents also a source for genetic diversity and genome plasticity in tomato. Furthermore, we identified processed break junctions pointing at the involvement of both homology directed and non‐homology directed repair pathways, suggesting a recombination machinery in tomato that is more complex than currently anticipated.
Original languageEnglish
Pages (from-to)480-492
JournalThe Plant Journal
Volume102
Issue number3
Early online date9 Dec 2019
DOIs
Publication statusPublished - May 2020

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