Caffeine solution was injected into the flower bud to recover F, fertility of the intersectional diploid Lilium species hybrid (2n=2x=24). 0.3% of caffeine solution was the most effective concentration to produce fertile 2n-gametes. The male and female gametes had a range of fertility following caffeine injection in relation to the different sizes of flower buds. The gametes from the treated OA-hybrids showed to be fertile. They were crossed both as male and female parents to the Asiatics, and produced 279 BC, progeny plants. Flowcytometric and chromosomal observation confirmed that all progenies were triploid (2n=3x=36), indicating that the 2n-gametes from the OA-hybrid were functional following caffeine treatment. There was no homoeologous recombination between parental species in the progeny derived from male gametes of the OA-hybrid. However, three plants originating from the female gametes showed homoeologous recombination between Oriental and Asiatic genomes. The functional female gametes were produced when treated at the range of 20-23mm and 34-37mm of flower bud length. Based on the chromosome constitution of the triploid BC1 progenies, it was concluded that FDR 2n-gamete was functional and homoeologous recombination had occurred during the origin of 2n gametes.
|Journal||Korean Journal of Genetics|
|Publication status||Published - 2005|
- lilium hybrids
- bc2 progenies
Lim, K. B., Barba Gonzalez, R., Shujun Zhou, Ramanna, M. S., & van Tuyl, J. M. (2005). Meiotic polyploidization with homoeologous recombination induced by caffeine treatment in interspecific lily hybrids. Korean Journal of Genetics, 27(3), 219-226. http://www.liliumbreeding.nl/LC%2010054.pdf