Meiotic polyploidization in Darwin hybrid tulips

A.R. Marasek Ciolakowska, S.L. Xie, M.S. Ramanna, P. Arens, J.M. van Tuyl

Research output: Chapter in Book/Report/Conference proceedingConference paper

3 Citations (Scopus)

Abstract

In our study, 2n gamete producing F1 hybrids of Darwin hybrids (T. gesneriana × T. fosteriana) have been selected and used as pollen donor with the aim of producing of polyploid tulips. In total, 308 one-year-old BC1 seedlings resulting from crosses between diploid and triploid T. gesneriana cultivars and diploid 2n gametes producers have been tested by flow cytometry analysis. Complementary, chromosome number and genome composition was evaluated for selected genotypes through genomic in situ hybridization (GISH) analysis. The progenies from crosses at diploid level (2x × 2x) were mostly diploids, whereas a few seedlings were triploids. In crosses 3x × 2x, 81 genotypes were tetraploids and 25 seedlings were pentaploids. Cytological analyses using GISH of the sexual polyploid progenies have shown considerable amounts of intergenomic recombination in the BC1 generations which is desirable for introgression breeding.
Original languageEnglish
Title of host publicationProceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide
EditorsT. Orlikowska
PublisherISHS
Pages187-192
Volume953
Publication statusPublished - 2012
EventThe 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide -
Duration: 2 Sep 20125 Sep 2012

Conference

ConferenceThe 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide
Period2/09/125/09/12

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Tulipa
diploidy
triploidy
in situ hybridization
germ cells
genomics
genotype
polyploidy
introgression
chromosome number
tetraploidy
flow cytometry
pollen
genome
breeding
cultivars

Cite this

Marasek Ciolakowska, A. R., Xie, S. L., Ramanna, M. S., Arens, P., & van Tuyl, J. M. (2012). Meiotic polyploidization in Darwin hybrid tulips. In T. Orlikowska (Ed.), Proceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide (Vol. 953, pp. 187-192). ISHS.
Marasek Ciolakowska, A.R. ; Xie, S.L. ; Ramanna, M.S. ; Arens, P. ; van Tuyl, J.M. / Meiotic polyploidization in Darwin hybrid tulips. Proceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide. editor / T. Orlikowska. Vol. 953 ISHS, 2012. pp. 187-192
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Marasek Ciolakowska, AR, Xie, SL, Ramanna, MS, Arens, P & van Tuyl, JM 2012, Meiotic polyploidization in Darwin hybrid tulips. in T Orlikowska (ed.), Proceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide. vol. 953, ISHS, pp. 187-192, The 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide, 2/09/12.

Meiotic polyploidization in Darwin hybrid tulips. / Marasek Ciolakowska, A.R.; Xie, S.L.; Ramanna, M.S.; Arens, P.; van Tuyl, J.M.

Proceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide. ed. / T. Orlikowska. Vol. 953 ISHS, 2012. p. 187-192.

Research output: Chapter in Book/Report/Conference proceedingConference paper

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Marasek Ciolakowska AR, Xie SL, Ramanna MS, Arens P, van Tuyl JM. Meiotic polyploidization in Darwin hybrid tulips. In Orlikowska T, editor, Proceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide. Vol. 953. ISHS. 2012. p. 187-192