Abstract
In our study, 2n gamete producing F1 hybrids of Darwin hybrids
(T. gesneriana × T. fosteriana) have been selected and used as pollen donor with the
aim of producing of polyploid tulips. In total, 308 one-year-old BC1 seedlings
resulting from crosses between diploid and triploid T. gesneriana cultivars and
diploid 2n gametes producers have been tested by flow cytometry analysis.
Complementary, chromosome number and genome composition was evaluated for
selected genotypes through genomic in situ hybridization (GISH) analysis. The
progenies from crosses at diploid level (2x × 2x) were mostly diploids, whereas a few
seedlings were triploids. In crosses 3x × 2x, 81 genotypes were tetraploids and 25
seedlings were pentaploids. Cytological analyses using GISH of the sexual polyploid
progenies have shown considerable amounts of intergenomic recombination in the
BC1 generations which is desirable for introgression breeding.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide |
| Editors | T. Orlikowska |
| Publisher | ISHS |
| Pages | 187-192 |
| Volume | 953 |
| Publication status | Published - 2012 |
| Event | The 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide - Duration: 2 Sept 2012 → 5 Sept 2012 |
Conference
| Conference | The 24th International Eucarpia Symposium Section Ornamentals “Ornamental breeding worldwide |
|---|---|
| Period | 2/09/12 → 5/09/12 |
Keywords
- 2n gametes
- Darwin hybrids
- Genomic in situ hybridization
- Interploidy crosses
- Tulipa