Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids.

S.A. Kamstra, J.H.S.G.M. de Jong, E. Jacobsen, M.S. Ramanna, A.G.J. Kuipers

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Chromosome association and chiasma formation were studied in pollen mother cells at metaphase I of four allotriplod BC1 plants (2n=3x=24) obtained from the backcross of the hybrid Alstroemeria aurea x A. inodora with its parent A. inodora. We distinguished the chromosomes of both parental species by genomic in situ hybridization (GISH), whereas the individual chromosomes were identified on the basis of their multicolour FISH banding patterns obtained after a second hybridization with two species-specific satellite repeats as probes. All the four BC1 plants possessed two genomes of A. inodora and one of A. aurea. Variable numbers of recombinant chromosomes, resulting from meiotic recombination in the interspecific hybrid, were present in these plants. The homologous A. inodora chromosomes generally formed bivalents, leaving the homoeologous A. aurea chromosomes unassociated. High frequencies of trivalents were observed for the chromosome sets that contained recombinant chromosomes, even when the recombinant segments were small. Chromosome associations in the trivalents were restricted to homologous segments. The implications of the absence of homoeologous chromosome pairing on gamete constitution and prospects for introgression in Alstroemeria are discussed.
Original languageEnglish
Pages (from-to)15-21
JournalHeredity
Volume93
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • in-situ hybridization
  • monosomic addition lines
  • backcross derivatives
  • somatic hybrids
  • distant hybrid
  • dna probes
  • recombination
  • plants
  • cytogenetics
  • progenies

Fingerprint Dive into the research topics of 'Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids.'. Together they form a unique fingerprint.

Cite this