Media for efficient generating nitrate- - nonutilizing (NIT) mutants of Verticillium dahliae, Colletotrichum gloeosporioides, Colletotrichum lindemuthianum and Fusarium oxysporum.

M. Rataj-Guranowska, K. Pieczul, E. Nowak, J.A. Hiemstra, M. Drapikowska

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    The effect of several media amended wit potassium chlorate (1.5% and 6%) on generation of nit mutants, especially nit M mutants from Verticillium dahliae, Colletotrichum gloeosporioides, Colletotrichum lindemuthianum and Fusarium oxysporum were studied. For all species minimal medium with 6% chlorate (MMC) was the most efficient for nit mutants recovery. Nit mutants were recovered with highest frequency from two Colletotrichum species and with the lowest frequency from F. oxysporum. Minimal medium with chlorate (MMC) was the most efficient for mit mutants recovery from Colletotrichum species, water agar medium with chlorate (WAC) for V. dahliae. MMC was as good as potato-dextrose-agar with chlorate (PDC) and corn-meal agar with chlorate (CMC) for the recovery of nit M mutants from F. oxysporum.
    Original languageEnglish
    Pages (from-to)95-105
    JournalPhytopathologia Polonica
    Volume22
    Publication statusPublished - 2001

    Keywords

    • nit mutant generation
    • media
    • Verticillium dahliae
    • Colletotrichum spp.

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