Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

Heleen T. Ouboter; Rob Mesman; Tom Sleutels; Jelle Postma; Martijn Wissink; Mike S.M. Jetten; Annemiek Ter Heijne; Tom Berben; Cornelia U. Welte

doi:10.1038/s41467-024-45758-2

Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

Heleen T. Ouboter, Rob Mesman, Tom Sleutels, Jelle Postma, Martijn Wissink, Mike S.M. Jetten, Annemiek Ter Heijne, Tom Berben, Cornelia U. Welte^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

40 Citations (Scopus)

Abstract

Anaerobic methanotrophic (ANME) archaea are environmentally important, uncultivated microorganisms that oxidize the potent greenhouse gas methane. During methane oxidation, ANME archaea engage in extracellular electron transfer (EET) with other microbes, metal oxides, and electrodes through unclear mechanisms. Here, we cultivate ANME-2d archaea (‘Ca. Methanoperedens’) in bioelectrochemical systems and observe strong methane-dependent current (91–93% of total current) associated with high enrichment of ‘Ca. Methanoperedens’ on the anode (up to 82% of the community), as determined by metagenomics and transmission electron microscopy. Electrochemical and metatranscriptomic analyses suggest that the EET mechanism is similar at various electrode potentials, with the possible involvement of an uncharacterized short-range electron transport protein complex and OmcZ nanowires.

Original language	English
Article number	1477
Journal	Nature Communications
Volume	15
DOIs	https://doi.org/10.1038/s41467-024-45758-2
Publication status	Published - 17 Feb 2024

Access to Document

10.1038/s41467-024-45758-2Licence: CC BY

https://edepot.wur.nl/651158Licence: CC BY

Electricity production by anaerobic methanotrophs
Ouboter, H. T. (Creator), Mesman, R. (Creator), Sleutels, T. (Creator), Postma, J. (Creator), Wissink, M. (Creator), Jetten, M. S. M. (Creator), Ter Heijne, A. (Creator), Berben, T. (Creator) & Welte, C. U. (Creator), Radboud University Nijmegen, 18 Aug 2023
https://www.ncbi.nlm.nih.gov/bioproject/995526
Dataset

Cite this

@article{b7d9330db4094584a582fe53f19677e4,

title = "Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea",

abstract = "Anaerobic methanotrophic (ANME) archaea are environmentally important, uncultivated microorganisms that oxidize the potent greenhouse gas methane. During methane oxidation, ANME archaea engage in extracellular electron transfer (EET) with other microbes, metal oxides, and electrodes through unclear mechanisms. Here, we cultivate ANME-2d archaea ({\textquoteleft}Ca. Methanoperedens{\textquoteright}) in bioelectrochemical systems and observe strong methane-dependent current (91–93\% of total current) associated with high enrichment of {\textquoteleft}Ca. Methanoperedens{\textquoteright} on the anode (up to 82\% of the community), as determined by metagenomics and transmission electron microscopy. Electrochemical and metatranscriptomic analyses suggest that the EET mechanism is similar at various electrode potentials, with the possible involvement of an uncharacterized short-range electron transport protein complex and OmcZ nanowires.",

author = "Ouboter, \{Heleen T.\} and Rob Mesman and Tom Sleutels and Jelle Postma and Martijn Wissink and Jetten, \{Mike S.M.\} and \{Ter Heijne\}, Annemiek and Tom Berben and Welte, \{Cornelia U.\}",

year = "2024",

month = feb,

day = "17",

doi = "10.1038/s41467-024-45758-2",

language = "English",

volume = "15",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature",

}

TY - JOUR

T1 - Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

AU - Ouboter, Heleen T.

AU - Mesman, Rob

AU - Sleutels, Tom

AU - Postma, Jelle

AU - Wissink, Martijn

AU - Jetten, Mike S.M.

AU - Ter Heijne, Annemiek

AU - Berben, Tom

AU - Welte, Cornelia U.

PY - 2024/2/17

Y1 - 2024/2/17

N2 - Anaerobic methanotrophic (ANME) archaea are environmentally important, uncultivated microorganisms that oxidize the potent greenhouse gas methane. During methane oxidation, ANME archaea engage in extracellular electron transfer (EET) with other microbes, metal oxides, and electrodes through unclear mechanisms. Here, we cultivate ANME-2d archaea (‘Ca. Methanoperedens’) in bioelectrochemical systems and observe strong methane-dependent current (91–93% of total current) associated with high enrichment of ‘Ca. Methanoperedens’ on the anode (up to 82% of the community), as determined by metagenomics and transmission electron microscopy. Electrochemical and metatranscriptomic analyses suggest that the EET mechanism is similar at various electrode potentials, with the possible involvement of an uncharacterized short-range electron transport protein complex and OmcZ nanowires.

AB - Anaerobic methanotrophic (ANME) archaea are environmentally important, uncultivated microorganisms that oxidize the potent greenhouse gas methane. During methane oxidation, ANME archaea engage in extracellular electron transfer (EET) with other microbes, metal oxides, and electrodes through unclear mechanisms. Here, we cultivate ANME-2d archaea (‘Ca. Methanoperedens’) in bioelectrochemical systems and observe strong methane-dependent current (91–93% of total current) associated with high enrichment of ‘Ca. Methanoperedens’ on the anode (up to 82% of the community), as determined by metagenomics and transmission electron microscopy. Electrochemical and metatranscriptomic analyses suggest that the EET mechanism is similar at various electrode potentials, with the possible involvement of an uncharacterized short-range electron transport protein complex and OmcZ nanowires.

U2 - 10.1038/s41467-024-45758-2

DO - 10.1038/s41467-024-45758-2

M3 - Article

C2 - 38368447

AN - SCOPUS:85185398463

SN - 2041-1723

VL - 15

JO - Nature Communications

JF - Nature Communications

M1 - 1477

ER -

Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

Abstract

Access to Document

Fingerprint

Datasets

Electricity production by anaerobic methanotrophs

Cite this