Mechanical transmission of poleroviruses

K. Hoffmann, M. Verbeek, A. Romano, A.M. Dullemans, J.F.J.M. van den Heuvel, F. van der Wilk

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30–50% systemically infected Nicotiana occidentalis P1 plants and 15–30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.
Original languageEnglish
Pages (from-to)197-201
JournalJournal of Virological Methods
Volume91
Issue number2
DOIs
Publication statusPublished - 2001

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Luteoviridae
Luteovirus
Solanum tuberosum
Tobacco
Biolistics
Viruses
Aphids
Viral RNA
Complementary DNA
RNA

Cite this

Hoffmann, K., Verbeek, M., Romano, A., Dullemans, A. M., van den Heuvel, J. F. J. M., & van der Wilk, F. (2001). Mechanical transmission of poleroviruses. Journal of Virological Methods, 91(2), 197-201. https://doi.org/10.1016/S0166-0934(00)00256-1
Hoffmann, K. ; Verbeek, M. ; Romano, A. ; Dullemans, A.M. ; van den Heuvel, J.F.J.M. ; van der Wilk, F. / Mechanical transmission of poleroviruses. In: Journal of Virological Methods. 2001 ; Vol. 91, No. 2. pp. 197-201.
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Hoffmann, K, Verbeek, M, Romano, A, Dullemans, AM, van den Heuvel, JFJM & van der Wilk, F 2001, 'Mechanical transmission of poleroviruses', Journal of Virological Methods, vol. 91, no. 2, pp. 197-201. https://doi.org/10.1016/S0166-0934(00)00256-1

Mechanical transmission of poleroviruses. / Hoffmann, K.; Verbeek, M.; Romano, A.; Dullemans, A.M.; van den Heuvel, J.F.J.M.; van der Wilk, F.

In: Journal of Virological Methods, Vol. 91, No. 2, 2001, p. 197-201.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Mechanical transmission of poleroviruses

AU - Hoffmann, K.

AU - Verbeek, M.

AU - Romano, A.

AU - Dullemans, A.M.

AU - van den Heuvel, J.F.J.M.

AU - van der Wilk, F.

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AB - Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30–50% systemically infected Nicotiana occidentalis P1 plants and 15–30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.

U2 - 10.1016/S0166-0934(00)00256-1

DO - 10.1016/S0166-0934(00)00256-1

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EP - 201

JO - Journal of Virological Methods

JF - Journal of Virological Methods

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