Mechanical transmission of poleroviruses

K. Hoffmann; M. Verbeek; A. Romano; A.M. Dullemans; J.F.J.M. van den Heuvel; F. van der Wilk

doi:10.1016/S0166-0934(00)00256-1

Mechanical transmission of poleroviruses

K. Hoffmann, M. Verbeek, A. Romano, A.M. Dullemans, J.F.J.M. van den Heuvel, F. van der Wilk

Biointeractions and Plant Health

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30–50% systemically infected Nicotiana occidentalis P1 plants and 15–30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.

Original language	English
Pages (from-to)	197-201
Journal	Journal of Virological Methods
Volume	91
Issue number	2
DOIs	https://doi.org/10.1016/S0166-0934(00)00256-1
Publication status	Published - 2001

Keywords

Biolistic inoculation
BWYV
Mechanical transmission
Particle bombardment
PLRV
Polerovirus

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10.1016/S0166-0934(00)00256-1

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title = "Mechanical transmission of poleroviruses",

abstract = "Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30–50% systemically infected Nicotiana occidentalis P1 plants and 15–30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.",

keywords = "Biolistic inoculation, BWYV, Mechanical transmission, Particle bombardment, PLRV, Polerovirus",

author = "K. Hoffmann and M. Verbeek and A. Romano and A.M. Dullemans and {van den Heuvel}, J.F.J.M. and {van der Wilk}, F.",

year = "2001",

doi = "10.1016/S0166-0934(00)00256-1",

language = "English",

volume = "91",

pages = "197--201",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

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TY - JOUR

T1 - Mechanical transmission of poleroviruses

AU - Hoffmann, K.

AU - Verbeek, M.

AU - Romano, A.

AU - Dullemans, A.M.

AU - van den Heuvel, J.F.J.M.

AU - van der Wilk, F.

PY - 2001

Y1 - 2001

N2 - Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30–50% systemically infected Nicotiana occidentalis P1 plants and 15–30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.

AB - Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30–50% systemically infected Nicotiana occidentalis P1 plants and 15–30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.

KW - Biolistic inoculation

KW - BWYV

KW - Mechanical transmission

KW - Particle bombardment

KW - PLRV

KW - Polerovirus

U2 - 10.1016/S0166-0934(00)00256-1

DO - 10.1016/S0166-0934(00)00256-1

M3 - Article

VL - 91

SP - 197

EP - 201

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 2

ER -

Mechanical transmission of poleroviruses

Abstract

Keywords

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