Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry

Steven M. Driever*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Oxygen is both product and substrate of photosynthesis and metabolism in plants, by oxygen evolution through water splitting and uptake by photorespiration and respiration. It is important to investigate these processes simultaneously in leaves, especially in response to environmental variables, such as light and temperature. To distinguish between processes that evolve or take up O2 in leaves in the light, in vivo gas exchange of stable isotopes of oxygen and membrane inlet mass spectrometry is used. A closed-cuvette system for gas exchange of leaf discs is described, using the stable isotopes 16O2 and 18O2, with a semi-permeable membrane gas inlet and isotope mass separation and detection by mass spectrometry. Measurement of evolution and uptake, as well as CO2 uptake, at a range of light levels allows composition of a light response curve, here described for French bean (Phaseolus vulgaris) and maize (Zea mays) leaf discs.

Original languageEnglish
Title of host publicationPhotosynthesis
Subtitle of host publicationMethods and Protocols
EditorsS. Covshoff
Place of PublicationNew York
PublisherHumana Press
Number of pages14
ISBN (Electronic)9781071637906
ISBN (Print)9781071637890, 9781071637920
Publication statusPublished - 23 Apr 2024

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • CO2 assimilation
  • Gas exchange
  • Membrane inlet mass spectrometry
  • MIMS
  • Oxygen evolution
  • Oxygen uptake
  • Photosynthesis
  • Stable isotopes


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