MC1R Genotype and Plumage Colouration in the Zebra Finch (Taeniopygia guttata): Population Structure Generates Artefactual Associations

J.L. Hoffman, E.T. Krause, K. Lehmann, O. Krüger

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Polymorphisms at the melanocortin-1 receptor (MC1R) gene have been linked to coloration in many vertebrate species. However, the potentially confounding influence of population structure has rarely been controlled for. We explored the role of the MC1R in a model avian system by sequencing the coding region in 162 zebra finches comprising 79 wild type and 83 white individuals from five stocks. Allelic counts differed significantly between the two plumage morphs at multiple segregating sites, but these were mostly synonymous. To provide a control, the birds were genotyped at eight microsatellites and subjected to Bayesian cluster analysis, revealing two distinct groups. We therefore crossed wild type with white individuals and backcrossed the F1s with white birds. No significant associations were detected in the resulting offspring, suggesting that our original findings were a byproduct of genome-wide divergence. Our results are consistent with a previous study that found no association between MC1R polymorphism and plumage coloration in leaf warblers. They also contribute towards a growing body of evidence suggesting that care should be taken to quantify, and where necessary control for, population structure in association studies.
Original languageEnglish
Article numbere86519
Number of pages9
JournalPLoS ONE
Volume9
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • genetic-basis
  • receptor gene
  • birds
  • polymorphism
  • evolution
  • mice
  • wild
  • ornaments
  • loci

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