Mating type sequences in asexually reproducing Fusarium species

Z. Kenényi, A. Moretti, C. Waalwijk, B. Oláh, L. Hornok

    Research output: Contribution to journalArticleAcademicpeer-review

    116 Citations (Scopus)

    Abstract

    To assess the potential for mating in several Fusarium species with no known sexual stage, we developed degenerate and semidegenerate oligonucleotide primers to identify conserved mating type (MAT) sequences in these fungi. The putative and high-mobility-group (HMG) box sequences from Fusarium avenaceum, F. culmorum, F. poae, and F. semitectum were compared to similar sequences that were described previously for other members of the genus. The DNA sequences of the regions flanking the amplified MAT regions were obtained by inverse PCR. These data were used to develop diagnostic primers suitable for the clear amplification of conserved mating type sequences from any member of the genus Fusarium. By using these diagnostic primers, we identified mating types of 122 strains belonging to 22 species of Fusarium. The box and the HMG box from the mating type genes are transcribed in F. avenaceum, F. culmorum, F. poae, and F. semitectum. The novelty of the PCR-based mating type identification system that we developed is that this method can be used on a wide range of Fusarium species, which have proven or expected teleomorphs in different ascomycetous genera, including Calonectria, Gibberella, and Nectria
    Original languageEnglish
    Pages (from-to)4419-4423
    JournalApplied and Environmental Microbiology
    Volume70
    Issue number8
    DOIs
    Publication statusPublished - 2004

    Keywords

    • gibberella-fujikuroi
    • vegetative compatibility
    • complex
    • genes
    • amplification
    • oxysporum
    • chemistry
    • genetics
    • biology
    • mutants

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