Mastoparan-Induced Cell Death Signalling in Chlamydomonas Reinhardtii

Z.P. Yordanova, V.M. Kapchina-Toteva, E.J. Woltering, S.M. Cristescu, F.J.M. Harren, E.T. Yakimova

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

The present study was focused on the elucidation of stress-induced cell death signaling events in the unicellular alga Chlamydomonas reinhardtii exposed to treatment with wasp venom mastoparan. By applying pharmacological approach with specific inhibitors, we have investigated the involvement of ethylene, nitric oxide and lipid signaling in MP-treated C. reinhardtii. Superior sensitive laser-based detectors were used for real-time measurement of trace amounts of ethylene and nitric oxide. The morphological features in the cells undergoing programmed cell death were detected by using laser-scanning confocal microscopy. Data showed that mastoparan induces programmed cell death in C. reinhardtii 137 C(+) that is associated with phospholipid signaling, including phospholipases C and D, ethylene and nitric oxide and, the dead cells express apoptotic-like cellular disintegration, involving cytoplasm shrinkage and condensation of the nucleus.
Original languageEnglish
Pages (from-to)730-734
JournalBiotechnology and Biotechnological Equipment
Volume23
Issue number2
DOIs
Publication statusPublished - 2009

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Chlamydomonas reinhardtii
Ethylene Oxide
Nitric Oxide
Cell Death
Wasp Venoms
Phospholipase D
Type C Phospholipases
Confocal Microscopy
Phospholipids
Cytoplasm
Lasers
Pharmacology
Lipids
mastoparan

Cite this

Yordanova, Z. P., Kapchina-Toteva, V. M., Woltering, E. J., Cristescu, S. M., Harren, F. J. M., & Yakimova, E. T. (2009). Mastoparan-Induced Cell Death Signalling in Chlamydomonas Reinhardtii. Biotechnology and Biotechnological Equipment, 23(2), 730-734. https://doi.org/10.1080/13102818.2009.10818528
Yordanova, Z.P. ; Kapchina-Toteva, V.M. ; Woltering, E.J. ; Cristescu, S.M. ; Harren, F.J.M. ; Yakimova, E.T. / Mastoparan-Induced Cell Death Signalling in Chlamydomonas Reinhardtii. In: Biotechnology and Biotechnological Equipment. 2009 ; Vol. 23, No. 2. pp. 730-734.
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Yordanova, ZP, Kapchina-Toteva, VM, Woltering, EJ, Cristescu, SM, Harren, FJM & Yakimova, ET 2009, 'Mastoparan-Induced Cell Death Signalling in Chlamydomonas Reinhardtii', Biotechnology and Biotechnological Equipment, vol. 23, no. 2, pp. 730-734. https://doi.org/10.1080/13102818.2009.10818528

Mastoparan-Induced Cell Death Signalling in Chlamydomonas Reinhardtii. / Yordanova, Z.P.; Kapchina-Toteva, V.M.; Woltering, E.J.; Cristescu, S.M.; Harren, F.J.M.; Yakimova, E.T.

In: Biotechnology and Biotechnological Equipment, Vol. 23, No. 2, 2009, p. 730-734.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Yordanova, Z.P.

AU - Kapchina-Toteva, V.M.

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AU - Yakimova, E.T.

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AB - The present study was focused on the elucidation of stress-induced cell death signaling events in the unicellular alga Chlamydomonas reinhardtii exposed to treatment with wasp venom mastoparan. By applying pharmacological approach with specific inhibitors, we have investigated the involvement of ethylene, nitric oxide and lipid signaling in MP-treated C. reinhardtii. Superior sensitive laser-based detectors were used for real-time measurement of trace amounts of ethylene and nitric oxide. The morphological features in the cells undergoing programmed cell death were detected by using laser-scanning confocal microscopy. Data showed that mastoparan induces programmed cell death in C. reinhardtii 137 C(+) that is associated with phospholipid signaling, including phospholipases C and D, ethylene and nitric oxide and, the dead cells express apoptotic-like cellular disintegration, involving cytoplasm shrinkage and condensation of the nucleus.

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