Mass spectrometry strategies for mycotoxins analysis in European beers

J. Rubert*, C. Soler, R. Marín, K.J. James, J. Mañes

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

In this work, an existent solid-phase extraction (SPE) procedure was used to study the occurrence of mycotoxins in different European beers. HPLC-QqQ-MS/MS and ultra high resolution mass analyser have been optimized for the analysis of 18 mycotoxins: the methods were validated according to the EU Commission Decision 2002/657/EC guidelines. In this sense, matrix-matched calibration was performed for each type of beer, obtaining an effective quantification. The recoveries ranged from 63 to 91% and repeatability and reproducibility expressed as relative standard deviation (RSD%) were lower than 17%. On one hand, HPLC-LTQ-Orbitrap ® was used for unambiguous identification of target mycotoxins, as well as for screening non-target mycotoxins, such as enniatins, fusaproliferin and deoxynivalenol-3-glucoside. On the other hand, the quantification was carried out using HPLC-QqQ-MS/MS instrument. At the end, Ochratoxin A, fumonisins, HT-2 and T-2 toxins were detected and quantified in European beers. Moreover, pale lager beer showed higher mycotoxin incidence than the other studied beers.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalFood Control
Volume30
Issue number1
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

Keywords

  • Beer
  • Mycotoxins
  • Orbitrap
  • SPE
  • Triple quadrupole

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