Marine neurotoxins: State of the art, bottlenecks, and perspectives for mode of action based methods of detection in seafood

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23 Citations (Scopus)

Abstract

Marine biotoxins can accumulate in fish and shellfish, representing a possible threat for consumers. Many marine biotoxins affect neuronal function essentially through their interaction with ion channels or receptors, leading to different symptoms including paralysis and even death. The detection of marine biotoxins in seafood products is therefore a priority. Official methods for control are often still using in vivo assays, such as the mouse bioassay. This test is considered unethical and the development of alternative assays is urgently required. Chemical analyses as well as in vitro assays have been developed to detect marine biotoxins in seafood. However, most of the current in vitro alternatives to animal testing present disadvantages: low throughput and lack of sensitivity resulting in a high number of false-negative results. Thus, there is an urgent need for the development of new in vitro tests that would allow the detection of marine biotoxins in seafood products at a low cost, with high throughput combined with high sensitivity, reproducibility, and predictivity. Mode of action based in vitro bioassays may provide tools that fulfil these requirements. This review covers the current state of the art of such mode of action based alternative assays to detect neurotoxic marine biotoxins in seafood.
LanguageEnglish
Pages87-100
JournalMolecular Nutrition & Food Research
Volume58
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Seafood
neurotoxins
Neurotoxins
seafoods
mechanism of action
assays
Biological Assay
Animal Testing Alternatives
bioassays
animal use alternatives
Shellfish
ion channels
paralysis
shellfish
methodology
Ion Channels
Paralysis
reproducibility
signs and symptoms (animals and humans)
control methods

Keywords

  • cerebellar granule neurons
  • paralytic shellfish toxins
  • receptor-binding assay
  • mouse taste cells
  • voltage-gated na+
  • domoic acid
  • sodium-channels
  • new-zealand
  • sandwich elisa
  • kainic acid

Cite this

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title = "Marine neurotoxins: State of the art, bottlenecks, and perspectives for mode of action based methods of detection in seafood",
abstract = "Marine biotoxins can accumulate in fish and shellfish, representing a possible threat for consumers. Many marine biotoxins affect neuronal function essentially through their interaction with ion channels or receptors, leading to different symptoms including paralysis and even death. The detection of marine biotoxins in seafood products is therefore a priority. Official methods for control are often still using in vivo assays, such as the mouse bioassay. This test is considered unethical and the development of alternative assays is urgently required. Chemical analyses as well as in vitro assays have been developed to detect marine biotoxins in seafood. However, most of the current in vitro alternatives to animal testing present disadvantages: low throughput and lack of sensitivity resulting in a high number of false-negative results. Thus, there is an urgent need for the development of new in vitro tests that would allow the detection of marine biotoxins in seafood products at a low cost, with high throughput combined with high sensitivity, reproducibility, and predictivity. Mode of action based in vitro bioassays may provide tools that fulfil these requirements. This review covers the current state of the art of such mode of action based alternative assays to detect neurotoxic marine biotoxins in seafood.",
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author = "J. Nicolas and P.J.M. Hendriksen and A. Gerssen and T.F.H. Bovee and I.M.C.M. Rietjens",
year = "2014",
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T1 - Marine neurotoxins: State of the art, bottlenecks, and perspectives for mode of action based methods of detection in seafood

AU - Nicolas, J.

AU - Hendriksen, P.J.M.

AU - Gerssen, A.

AU - Bovee, T.F.H.

AU - Rietjens, I.M.C.M.

PY - 2014

Y1 - 2014

N2 - Marine biotoxins can accumulate in fish and shellfish, representing a possible threat for consumers. Many marine biotoxins affect neuronal function essentially through their interaction with ion channels or receptors, leading to different symptoms including paralysis and even death. The detection of marine biotoxins in seafood products is therefore a priority. Official methods for control are often still using in vivo assays, such as the mouse bioassay. This test is considered unethical and the development of alternative assays is urgently required. Chemical analyses as well as in vitro assays have been developed to detect marine biotoxins in seafood. However, most of the current in vitro alternatives to animal testing present disadvantages: low throughput and lack of sensitivity resulting in a high number of false-negative results. Thus, there is an urgent need for the development of new in vitro tests that would allow the detection of marine biotoxins in seafood products at a low cost, with high throughput combined with high sensitivity, reproducibility, and predictivity. Mode of action based in vitro bioassays may provide tools that fulfil these requirements. This review covers the current state of the art of such mode of action based alternative assays to detect neurotoxic marine biotoxins in seafood.

AB - Marine biotoxins can accumulate in fish and shellfish, representing a possible threat for consumers. Many marine biotoxins affect neuronal function essentially through their interaction with ion channels or receptors, leading to different symptoms including paralysis and even death. The detection of marine biotoxins in seafood products is therefore a priority. Official methods for control are often still using in vivo assays, such as the mouse bioassay. This test is considered unethical and the development of alternative assays is urgently required. Chemical analyses as well as in vitro assays have been developed to detect marine biotoxins in seafood. However, most of the current in vitro alternatives to animal testing present disadvantages: low throughput and lack of sensitivity resulting in a high number of false-negative results. Thus, there is an urgent need for the development of new in vitro tests that would allow the detection of marine biotoxins in seafood products at a low cost, with high throughput combined with high sensitivity, reproducibility, and predictivity. Mode of action based in vitro bioassays may provide tools that fulfil these requirements. This review covers the current state of the art of such mode of action based alternative assays to detect neurotoxic marine biotoxins in seafood.

KW - cerebellar granule neurons

KW - paralytic shellfish toxins

KW - receptor-binding assay

KW - mouse taste cells

KW - voltage-gated na+

KW - domoic acid

KW - sodium-channels

KW - new-zealand

KW - sandwich elisa

KW - kainic acid

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DO - 10.1002/mnfr.201300520

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VL - 58

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JO - Molecular Nutrition & Food Research

T2 - Molecular Nutrition & Food Research

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SN - 1613-4125

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