Marek’s disease virus infection of natural killer cells

Luca D. Bertzbach, Daphne A. van Haarlem, Sonja Härtle, Benedikt B. Kaufer*, Christine A. Jansen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)


Natural killer (NK) cells are key players in the innate immune response. They kill virus-infected cells and are crucial for the induction of adaptive immune responses. Marek's disease virus (MDV) is a highly contagious alphaherpesvirus that causes deadly T cell lymphomas in chickens. Host resistance to MDV is associated with differences in NK cell responses; however, the exact role of NK cells in the control of MDV remains unknown. In this study, we assessed if MDV can infect NK cells and alter their activation. Surprisingly, we could demonstrate that primary chicken NK cells are very efficiently infected with very virulent RB-1B MDV and the live-attenuated CVI988 vaccine. Flow cytometry analysis revealed that both RB-1B and CVI988 enhance NK cell degranulation and increase interferon gamma (IFNγ) production in vitro. In addition, we could show that the MDV Eco Q-encoded oncogene (meq) contributes to the induction of NK cell activation using meq knockout viruses. Taken together, our data revealed for the first time that NK cells are efficiently infectable with MDV and that this oncogenic alphaherpesvirus enhances NK cell degranulation and increased IFNγ production in vitro.
Original languageEnglish
Article number588
Issue number12
Publication statusPublished - Dec 2019
Externally publishedYes


  • CD107
  • Cell tropism
  • Chickens
  • CVI988
  • Interferon gamma (IFNγ)
  • Meq
  • NK cells
  • RB-1B


Dive into the research topics of 'Marek’s disease virus infection of natural killer cells'. Together they form a unique fingerprint.

Cite this