Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii

C.M. Kreike

Research output: Thesisexternal PhD, WU

Abstract

This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Heyting, C., Promotor
  • Stiekema, W.J., Promotor, External person
Award date28 Jun 1995
Place of PublicationWageningen
Publisher
Print ISBNs9789054854081
DOIs
Publication statusPublished - 28 Jun 1995

Keywords

  • solanum tuberosum
  • potatoes
  • plant pests
  • pratylenchus
  • heteroderidae
  • tylenchidae
  • chromosome maps
  • gene mapping
  • genetic engineering
  • recombinant dna
  • plant breeding
  • disease resistance
  • pest resistance
  • plants
  • globodera

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