Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii

C.M. Kreike

Research output: Thesisexternal PhD, WU

Abstract

<p>This thesis describes the mapping and characterisation of QTLs, in <em>Solanum spegazzinii,</em> that are involved in resistance to the potato cyst-nematodes <em>Globodera rostochiensis</em> and <em>G. pallida.</em> For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to <em>G.</em><em>rostochiensis</em> and one major QTL that is involved in resistance to <em>G.</em><em>pallida.</em> Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Heyting, C., Promotor
  • Stiekema, W.J., Promotor, External person
Award date28 Jun 1995
Place of PublicationS.l.
Publisher
Print ISBNs9789054854081
Publication statusPublished - 1995

Keywords

  • solanum tuberosum
  • potatoes
  • plant pests
  • pratylenchus
  • heteroderidae
  • tylenchidae
  • chromosome maps
  • gene mapping
  • genetic engineering
  • recombinant dna
  • plant breeding
  • disease resistance
  • pest resistance
  • plants
  • globodera

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