Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii

C.M. Kreike

Research output: Thesisexternal PhD, WUAcademic

Abstract

<p>This thesis describes the mapping and characterisation of QTLs, in <em>Solanum spegazzinii,</em> that are involved in resistance to the potato cyst-nematodes <em>Globodera rostochiensis</em> and <em>G. pallida.</em> For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to <em>G.</em><em>rostochiensis</em> and one major QTL that is involved in resistance to <em>G.</em><em>pallida.</em> Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Heyting, C., Promotor
  • Stiekema, W.J., Promotor, External person
Award date28 Jun 1995
Place of PublicationS.l.
Publisher
Print ISBNs9789054854081
Publication statusPublished - 1995

Fingerprint

Solanum brevicaule
quantitative trait loci
Nematoda
potatoes
Globodera pallida
Globodera rostochiensis
cyst nematodes
DNA
quantitative traits
chromosome mapping
linkage (genetics)
restriction fragment length polymorphism
tubers

Keywords

  • solanum tuberosum
  • potatoes
  • plant pests
  • pratylenchus
  • heteroderidae
  • tylenchidae
  • chromosome maps
  • gene mapping
  • genetic engineering
  • recombinant dna
  • plant breeding
  • disease resistance
  • pest resistance
  • plants
  • globodera

Cite this

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title = "Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii",
abstract = "This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.",
keywords = "solanum tuberosum, aardappelen, plantenplagen, pratylenchus, heteroderidae, tylenchidae, chromosoomkaarten, genkartering, genetische modificatie, recombinant dna, plantenveredeling, ziekteresistentie, plaagresistentie, planten, globodera, solanum tuberosum, potatoes, plant pests, pratylenchus, heteroderidae, tylenchidae, chromosome maps, gene mapping, genetic engineering, recombinant dna, plant breeding, disease resistance, pest resistance, plants, globodera",
author = "C.M. Kreike",
note = "WU thesis 1963 Proefschrift Wageningen",
year = "1995",
language = "English",
isbn = "9789054854081",
publisher = "Kreike",

}

Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii. / Kreike, C.M.

S.l. : Kreike, 1995. 103 p.

Research output: Thesisexternal PhD, WUAcademic

TY - THES

T1 - Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii

AU - Kreike, C.M.

N1 - WU thesis 1963 Proefschrift Wageningen

PY - 1995

Y1 - 1995

N2 - This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.

AB - This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.

KW - solanum tuberosum

KW - aardappelen

KW - plantenplagen

KW - pratylenchus

KW - heteroderidae

KW - tylenchidae

KW - chromosoomkaarten

KW - genkartering

KW - genetische modificatie

KW - recombinant dna

KW - plantenveredeling

KW - ziekteresistentie

KW - plaagresistentie

KW - planten

KW - globodera

KW - solanum tuberosum

KW - potatoes

KW - plant pests

KW - pratylenchus

KW - heteroderidae

KW - tylenchidae

KW - chromosome maps

KW - gene mapping

KW - genetic engineering

KW - recombinant dna

KW - plant breeding

KW - disease resistance

KW - pest resistance

KW - plants

KW - globodera

M3 - external PhD, WU

SN - 9789054854081

PB - Kreike

CY - S.l.

ER -