Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium

G. Pretzer, J. van der Meulen, J. Snel, R. van der Meer, M. Kleerebezem, Th. Niewold, M.M. Hulst, M.A. Smits

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35 Citations (Scopus)

Abstract

Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8¿h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8¿h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
Original languageEnglish
Pages (from-to)215-223
JournalFEMS Immunology and Medical Microbiology
Volume54
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • enterotoxigenic escherichia-coli
  • human intestinal-mucosa
  • acute-pancreatitis
  • net absorption
  • rat intestine
  • immune-system
  • reg-i
  • expression
  • bacteria
  • adhesion

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