Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA

V.J. Carrion, M. van der Voort, E. Arrebola, J.A. Gutiérrez-Barranquero, A. de Vicente, J.M. Raaijmakers, F.M. Cazorla

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Background The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. Results The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. Conclusions From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis.
Original languageEnglish
Article number46
Number of pages13
JournalBMC Microbiology
Volume14
DOIs
Publication statusPublished - 2014

Keywords

  • signal-transduction pathway
  • artemis comparison tool
  • 2-component system
  • response regulator
  • escherichia-coli
  • fluorescens pf-5
  • rna recognition
  • tomato dc3000
  • virulence
  • gaca

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