Manganese accumulation and tissue-level distribution in Australian Macadamia (Proteaceae) species

Farida Abubakari; Philip Nti Nkrumah; Denise R. Fernando; Peter D. Erskine; Antony van der Ent

doi:10.1016/j.envexpbot.2021.104668

Manganese accumulation and tissue-level distribution in Australian Macadamia (Proteaceae) species

Farida Abubakari, Philip Nti Nkrumah, Denise R. Fernando, Peter D. Erskine, Antony van der Ent^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Background and aims: Australian Macadamia species are known for their edible nuts as well as their ability to take up high concentrations of manganese (Mn) into their leaves. However, little is known about the ecophysiology of Mn in species of Macadamia. Methods: This study investigated (i) Mn accumulation in wild Macadamia using handheld X-ray fluorescence spectroscopy (XRF) of herbarium collections, and (ii) Mn uptake in three out of four known Macadamia species (M. tetraphylla, M. integrifolia and M. ternifolia) in a pot experiment that delivered Mn treatments in both solid (as natural Mn-minerals mixed with quartz sand) and liquid form (as MnSO₄ solution added to soil). Tissue samples were analysed for bulk elemental concentrations and for in vivo distribution of Mn and other elements using laboratory μXRF analysis. Key results: The results revealed that wild M. ternifolia and M. integrifolia are strong Mn accumulators with up to 9600 μg Mn g⁻¹ and 8500 μg Mn g^-1, respectively, followed by M. jansenii (6400 μg Mn g^-1) and M. tetraphylla (5100 μg Mn g^-1). Under the controlled conditions, high Mn accumulation was demonstrated for M. integrifolia and M. tetraphylla with greater than 7500 μg Mn g⁻¹ in mature leaves in the highest Mn treatment level. Our results further showed that Mn was efficiently translocated from the roots to the shoots with translocation factor >1 in the studied species. The laboratory μXRF analysis revealed that Mn was absent from the vascular bundles and concentrated in the areas between veins. Conclusions: All the four Macadamia species have high foliar Mn concentrations qualifying them as strong Mn accumulators in nature as determined by handheld XRF analysis of herbarium specimens. Under experimental conditions, M. integrifolia and M. tetraphylla were confirmed to be strong Mn accumulators. Manganese was shown by laboratory μXRF analysis to be concentrated in the inter-veinal areas of the leaf lamina.

Original language	English
Article number	104668
Journal	Environmental and Experimental Botany
Volume	193
DOIs	https://doi.org/10.1016/j.envexpbot.2021.104668
Publication status	Published - Jan 2022
Externally published	Yes

Keywords

Elemental distribution
Hyperaccumulator
Macadamia
Nuts
Translocation

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10.1016/j.envexpbot.2021.104668

Cite this

@article{ed123d3a389c4b4da1acfb944d7471db,

title = "Manganese accumulation and tissue-level distribution in Australian Macadamia (Proteaceae) species",

abstract = "Background and aims: Australian Macadamia species are known for their edible nuts as well as their ability to take up high concentrations of manganese (Mn) into their leaves. However, little is known about the ecophysiology of Mn in species of Macadamia. Methods: This study investigated (i) Mn accumulation in wild Macadamia using handheld X-ray fluorescence spectroscopy (XRF) of herbarium collections, and (ii) Mn uptake in three out of four known Macadamia species (M. tetraphylla, M. integrifolia and M. ternifolia) in a pot experiment that delivered Mn treatments in both solid (as natural Mn-minerals mixed with quartz sand) and liquid form (as MnSO4 solution added to soil). Tissue samples were analysed for bulk elemental concentrations and for in vivo distribution of Mn and other elements using laboratory μXRF analysis. Key results: The results revealed that wild M. ternifolia and M. integrifolia are strong Mn accumulators with up to 9600 μg Mn g−1 and 8500 μg Mn g-1, respectively, followed by M. jansenii (6400 μg Mn g-1) and M. tetraphylla (5100 μg Mn g-1). Under the controlled conditions, high Mn accumulation was demonstrated for M. integrifolia and M. tetraphylla with greater than 7500 μg Mn g−1 in mature leaves in the highest Mn treatment level. Our results further showed that Mn was efficiently translocated from the roots to the shoots with translocation factor >1 in the studied species. The laboratory μXRF analysis revealed that Mn was absent from the vascular bundles and concentrated in the areas between veins. Conclusions: All the four Macadamia species have high foliar Mn concentrations qualifying them as strong Mn accumulators in nature as determined by handheld XRF analysis of herbarium specimens. Under experimental conditions, M. integrifolia and M. tetraphylla were confirmed to be strong Mn accumulators. Manganese was shown by laboratory μXRF analysis to be concentrated in the inter-veinal areas of the leaf lamina.",

keywords = "Elemental distribution, Hyperaccumulator, Macadamia, Nuts, Translocation",

author = "Farida Abubakari and Nkrumah, {Philip Nti} and Fernando, {Denise R.} and Erskine, {Peter D.} and {van der Ent}, Antony",

year = "2022",

month = jan,

doi = "10.1016/j.envexpbot.2021.104668",

language = "English",

volume = "193",

journal = "Environmental and Experimental Botany",

issn = "0098-8472",

publisher = "Elsevier",

}

TY - JOUR

T1 - Manganese accumulation and tissue-level distribution in Australian Macadamia (Proteaceae) species

AU - Abubakari, Farida

AU - Nkrumah, Philip Nti

AU - Fernando, Denise R.

AU - Erskine, Peter D.

AU - van der Ent, Antony

PY - 2022/1

Y1 - 2022/1

N2 - Background and aims: Australian Macadamia species are known for their edible nuts as well as their ability to take up high concentrations of manganese (Mn) into their leaves. However, little is known about the ecophysiology of Mn in species of Macadamia. Methods: This study investigated (i) Mn accumulation in wild Macadamia using handheld X-ray fluorescence spectroscopy (XRF) of herbarium collections, and (ii) Mn uptake in three out of four known Macadamia species (M. tetraphylla, M. integrifolia and M. ternifolia) in a pot experiment that delivered Mn treatments in both solid (as natural Mn-minerals mixed with quartz sand) and liquid form (as MnSO4 solution added to soil). Tissue samples were analysed for bulk elemental concentrations and for in vivo distribution of Mn and other elements using laboratory μXRF analysis. Key results: The results revealed that wild M. ternifolia and M. integrifolia are strong Mn accumulators with up to 9600 μg Mn g−1 and 8500 μg Mn g-1, respectively, followed by M. jansenii (6400 μg Mn g-1) and M. tetraphylla (5100 μg Mn g-1). Under the controlled conditions, high Mn accumulation was demonstrated for M. integrifolia and M. tetraphylla with greater than 7500 μg Mn g−1 in mature leaves in the highest Mn treatment level. Our results further showed that Mn was efficiently translocated from the roots to the shoots with translocation factor >1 in the studied species. The laboratory μXRF analysis revealed that Mn was absent from the vascular bundles and concentrated in the areas between veins. Conclusions: All the four Macadamia species have high foliar Mn concentrations qualifying them as strong Mn accumulators in nature as determined by handheld XRF analysis of herbarium specimens. Under experimental conditions, M. integrifolia and M. tetraphylla were confirmed to be strong Mn accumulators. Manganese was shown by laboratory μXRF analysis to be concentrated in the inter-veinal areas of the leaf lamina.

AB - Background and aims: Australian Macadamia species are known for their edible nuts as well as their ability to take up high concentrations of manganese (Mn) into their leaves. However, little is known about the ecophysiology of Mn in species of Macadamia. Methods: This study investigated (i) Mn accumulation in wild Macadamia using handheld X-ray fluorescence spectroscopy (XRF) of herbarium collections, and (ii) Mn uptake in three out of four known Macadamia species (M. tetraphylla, M. integrifolia and M. ternifolia) in a pot experiment that delivered Mn treatments in both solid (as natural Mn-minerals mixed with quartz sand) and liquid form (as MnSO4 solution added to soil). Tissue samples were analysed for bulk elemental concentrations and for in vivo distribution of Mn and other elements using laboratory μXRF analysis. Key results: The results revealed that wild M. ternifolia and M. integrifolia are strong Mn accumulators with up to 9600 μg Mn g−1 and 8500 μg Mn g-1, respectively, followed by M. jansenii (6400 μg Mn g-1) and M. tetraphylla (5100 μg Mn g-1). Under the controlled conditions, high Mn accumulation was demonstrated for M. integrifolia and M. tetraphylla with greater than 7500 μg Mn g−1 in mature leaves in the highest Mn treatment level. Our results further showed that Mn was efficiently translocated from the roots to the shoots with translocation factor >1 in the studied species. The laboratory μXRF analysis revealed that Mn was absent from the vascular bundles and concentrated in the areas between veins. Conclusions: All the four Macadamia species have high foliar Mn concentrations qualifying them as strong Mn accumulators in nature as determined by handheld XRF analysis of herbarium specimens. Under experimental conditions, M. integrifolia and M. tetraphylla were confirmed to be strong Mn accumulators. Manganese was shown by laboratory μXRF analysis to be concentrated in the inter-veinal areas of the leaf lamina.

KW - Elemental distribution

KW - Hyperaccumulator

KW - Macadamia

KW - Nuts

KW - Translocation

U2 - 10.1016/j.envexpbot.2021.104668

DO - 10.1016/j.envexpbot.2021.104668

M3 - Article

AN - SCOPUS:85118546963

SN - 0098-8472

VL - 193

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

M1 - 104668

ER -

Manganese accumulation and tissue-level distribution in Australian Macadamia (Proteaceae) species

Abstract

Keywords

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