Maize resistance to witchweed through changes in strigolactone biosynthesis

C. Li; L. Dong; J. Durairaj; J.C. Guan; M. Yoshimura; P. Quinodoz; R. Horber; K. Gaus; J. Li; Y.B. Setotaw; J. Qi; H. De Groote; Y. Wang; B. Thiombiano; K. Floková; A. Walmsley; T.V. Charnikhova; A. Chojnacka; S. Correia de Lemos; Y. Ding; D. Skibbe; K. Hermann; C. Screpanti; A. De Mesmaeker; E.A. Schmelz; A. Menkir; M. Medema; A.D.J. Van Dijk; J. Wu; K.E. Koch; H.J. Bouwmeester

doi:10.1126/science.abq4775

Maize resistance to witchweed through changes in strigolactone biosynthesis

C. Li, L. Dong^*, J. Durairaj, J.C. Guan, M. Yoshimura, P. Quinodoz, R. Horber, K. Gaus, J. Li, Y.B. Setotaw, J. Qi, H. De Groote, Y. Wang, B. Thiombiano, K. Floková, A. Walmsley, T.V. Charnikhova, A. Chojnacka, S. Correia de Lemos, Y. DingD. Skibbe, K. Hermann, C. Screpanti, A. De Mesmaeker, E.A. Schmelz, A. Menkir, M. Medema, A.D.J. Van Dijk, J. Wu, K.E. Koch, H.J. Bouwmeester^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

Maize (Zea mays) is a major staple crop in Africa, where its yield and the livelihood of millions are compromised by the parasitic witchweed Striga. Germination of Striga is induced by strigolactones exuded from maize roots into the rhizosphere. In a maize germplasm collection, we identified two strigolactones, zealactol and zealactonoic acid, which stimulate less Striga germination than the major maize strigolactone, zealactone. We then showed that a single cytochrome P450, ZmCYP706C37, catalyzes a series of oxidative steps in the maize-strigolactone biosynthetic pathway. Reduction in activity of this enzyme and two others involved in the pathway, ZmMAX1b and ZmCLAMT1, can change strigolactone composition and reduce Striga germination and infection. These results offer prospects for breeding Striga-resistant maize.

Original language	English
Pages (from-to)	94-99
Journal	Science (New York, N.Y.)
Volume	379
Issue number	6627
DOIs	https://doi.org/10.1126/science.abq4775
Publication status	Published - 5 Jan 2023

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Li, C., Dong, L., Durairaj, J., Guan, J. C., Yoshimura, M., Quinodoz, P., Horber, R., Gaus, K., Li, J., Setotaw, Y. B., Qi, J., De Groote, H., Wang, Y., Thiombiano, B., Floková, K., Walmsley, A., Charnikhova, T. V., Chojnacka, A., Correia de Lemos, S., ... Bouwmeester, H. J. (2023). Maize resistance to witchweed through changes in strigolactone biosynthesis. Science (New York, N.Y.), 379(6627), 94-99. https://doi.org/10.1126/science.abq4775

@article{6862f1d29a9444a4977ebf6ae6ac161b,

title = "Maize resistance to witchweed through changes in strigolactone biosynthesis",

abstract = "Maize (Zea mays) is a major staple crop in Africa, where its yield and the livelihood of millions are compromised by the parasitic witchweed Striga. Germination of Striga is induced by strigolactones exuded from maize roots into the rhizosphere. In a maize germplasm collection, we identified two strigolactones, zealactol and zealactonoic acid, which stimulate less Striga germination than the major maize strigolactone, zealactone. We then showed that a single cytochrome P450, ZmCYP706C37, catalyzes a series of oxidative steps in the maize-strigolactone biosynthetic pathway. Reduction in activity of this enzyme and two others involved in the pathway, ZmMAX1b and ZmCLAMT1, can change strigolactone composition and reduce Striga germination and infection. These results offer prospects for breeding Striga-resistant maize.",

author = "C. Li and L. Dong and J. Durairaj and J.C. Guan and M. Yoshimura and P. Quinodoz and R. Horber and K. Gaus and J. Li and Y.B. Setotaw and J. Qi and {De Groote}, H. and Y. Wang and B. Thiombiano and K. Flokov{\'a} and A. Walmsley and T.V. Charnikhova and A. Chojnacka and {Correia de Lemos}, S. and Y. Ding and D. Skibbe and K. Hermann and C. Screpanti and {De Mesmaeker}, A. and E.A. Schmelz and A. Menkir and M. Medema and {Van Dijk}, A.D.J. and J. Wu and K.E. Koch and H.J. Bouwmeester",

year = "2023",

month = jan,

day = "5",

doi = "10.1126/science.abq4775",

language = "English",

volume = "379",

pages = "94--99",

journal = "Science (New York, N.Y.)",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6627",

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Li, C, Dong, L, Durairaj, J, Guan, JC, Yoshimura, M, Quinodoz, P, Horber, R, Gaus, K, Li, J, Setotaw, YB, Qi, J, De Groote, H, Wang, Y, Thiombiano, B, Floková, K, Walmsley, A, Charnikhova, TV, Chojnacka, A, Correia de Lemos, S, Ding, Y, Skibbe, D, Hermann, K, Screpanti, C, De Mesmaeker, A, Schmelz, EA, Menkir, A, Medema, M , Van Dijk, ADJ, Wu, J, Koch, KE & Bouwmeester, HJ 2023, 'Maize resistance to witchweed through changes in strigolactone biosynthesis', Science (New York, N.Y.), vol. 379, no. 6627, pp. 94-99. https://doi.org/10.1126/science.abq4775

TY - JOUR

T1 - Maize resistance to witchweed through changes in strigolactone biosynthesis

AU - Li, C.

AU - Dong, L.

AU - Durairaj, J.

AU - Guan, J.C.

AU - Yoshimura, M.

AU - Quinodoz, P.

AU - Horber, R.

AU - Gaus, K.

AU - Li, J.

AU - Setotaw, Y.B.

AU - Qi, J.

AU - De Groote, H.

AU - Wang, Y.

AU - Thiombiano, B.

AU - Floková, K.

AU - Walmsley, A.

AU - Charnikhova, T.V.

AU - Chojnacka, A.

AU - Correia de Lemos, S.

AU - Ding, Y.

AU - Skibbe, D.

AU - Hermann, K.

AU - Screpanti, C.

AU - De Mesmaeker, A.

AU - Schmelz, E.A.

AU - Menkir, A.

AU - Medema, M.

AU - Van Dijk, A.D.J.

AU - Wu, J.

AU - Koch, K.E.

AU - Bouwmeester, H.J.

PY - 2023/1/5

Y1 - 2023/1/5

N2 - Maize (Zea mays) is a major staple crop in Africa, where its yield and the livelihood of millions are compromised by the parasitic witchweed Striga. Germination of Striga is induced by strigolactones exuded from maize roots into the rhizosphere. In a maize germplasm collection, we identified two strigolactones, zealactol and zealactonoic acid, which stimulate less Striga germination than the major maize strigolactone, zealactone. We then showed that a single cytochrome P450, ZmCYP706C37, catalyzes a series of oxidative steps in the maize-strigolactone biosynthetic pathway. Reduction in activity of this enzyme and two others involved in the pathway, ZmMAX1b and ZmCLAMT1, can change strigolactone composition and reduce Striga germination and infection. These results offer prospects for breeding Striga-resistant maize.

AB - Maize (Zea mays) is a major staple crop in Africa, where its yield and the livelihood of millions are compromised by the parasitic witchweed Striga. Germination of Striga is induced by strigolactones exuded from maize roots into the rhizosphere. In a maize germplasm collection, we identified two strigolactones, zealactol and zealactonoic acid, which stimulate less Striga germination than the major maize strigolactone, zealactone. We then showed that a single cytochrome P450, ZmCYP706C37, catalyzes a series of oxidative steps in the maize-strigolactone biosynthetic pathway. Reduction in activity of this enzyme and two others involved in the pathway, ZmMAX1b and ZmCLAMT1, can change strigolactone composition and reduce Striga germination and infection. These results offer prospects for breeding Striga-resistant maize.

U2 - 10.1126/science.abq4775

DO - 10.1126/science.abq4775

M3 - Article

C2 - 36603079

AN - SCOPUS:85145645793

SN - 0036-8075

VL - 379

SP - 94

EP - 99

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 6627

ER -

Maize resistance to witchweed through changes in strigolactone biosynthesis

Abstract

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