MADS evolution : insights into evolutionary changes in transcription factors and their binding sites

Suze-Annigje de Bruijn

Research output: Thesisinternal PhD, WUAcademic

Abstract

Although most flowers follow a conserved 'bauplan' consisting of sepals, petals, stamens and carpels, there is a remarkable amount of morphological diversity. Interestingly, all flowers are specified by the conserved (A)BCE-model. Most of the transcription factors in this model belong to the MADS-domain family. We examined how these transcription factors and their binding sites in the genome evolved, as a first step to elucidate how diversity in flower morphology has been created.

We analyzed the evolution of transcription factor binding sites by comparing binding sites of the major floral regulator SEPALLATA3 between two closely related Arabidopsis species, as well as between A. thaliana ecotypes. We found substantial overlap in transcription factor binding profiles between ecotypes, but limited overlap between the related species.

We also assessed how transcription factors themselves can change in their properties by analyzing the divergence between paralogs. We examined how the PISTILLATA paralogs in Tarenaya hassleriana diverged, as this species occupies an interesting position in the eudicot phylogeny. We also studied whether divergence of the APETALA3 paralogs in Aquilegia could explain the specification of an additional floral organ in this genus.  In both cases, we conclude that the paralogs diverged from each other in their biochemical properties.

In the future, it would be interesting to assess how these changes in transcription factors and their binding sites affect floral regulatory networks and ultimately floral shape.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Angenent, Gerco, Promotor
  • Kaufmann, K., Co-promotor, External person
Award date20 Nov 2017
Place of PublicationWageningen
Publisher
Print ISBNs9789463436700
DOIs
Publication statusPublished - 2017

Fingerprint

binding sites
transcription factors
ecotypes
flowers
Aquilegia
carpels
stamens
calyx
corolla
Arabidopsis thaliana
Arabidopsis
genome
phylogeny

Keywords

  • plants
  • evolution
  • mads-box proteins
  • transcription factors
  • flowers
  • molecular biology

Cite this

de Bruijn, Suze-Annigje. / MADS evolution : insights into evolutionary changes in transcription factors and their binding sites. Wageningen : Wageningen University, 2017. 195 p.
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title = "MADS evolution : insights into evolutionary changes in transcription factors and their binding sites",
abstract = "Although most flowers follow a conserved 'bauplan' consisting of sepals, petals, stamens and carpels, there is a remarkable amount of morphological diversity. Interestingly, all flowers are specified by the conserved (A)BCE-model. Most of the transcription factors in this model belong to the MADS-domain family. We examined how these transcription factors and their binding sites in the genome evolved, as a first step to elucidate how diversity in flower morphology has been created. We analyzed the evolution of transcription factor binding sites by comparing binding sites of the major floral regulator SEPALLATA3 between two closely related Arabidopsis species, as well as between A. thaliana ecotypes. We found substantial overlap in transcription factor binding profiles between ecotypes, but limited overlap between the related species. We also assessed how transcription factors themselves can change in their properties by analyzing the divergence between paralogs. We examined how the PISTILLATA paralogs in Tarenaya hassleriana diverged, as this species occupies an interesting position in the eudicot phylogeny. We also studied whether divergence of the APETALA3 paralogs in Aquilegia could explain the specification of an additional floral organ in this genus.  In both cases, we conclude that the paralogs diverged from each other in their biochemical properties. In the future, it would be interesting to assess how these changes in transcription factors and their binding sites affect floral regulatory networks and ultimately floral shape.",
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MADS evolution : insights into evolutionary changes in transcription factors and their binding sites. / de Bruijn, Suze-Annigje.

Wageningen : Wageningen University, 2017. 195 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - MADS evolution : insights into evolutionary changes in transcription factors and their binding sites

AU - de Bruijn, Suze-Annigje

N1 - WU thesis 6812 Includes bibliographical references. - With summary in English

PY - 2017

Y1 - 2017

N2 - Although most flowers follow a conserved 'bauplan' consisting of sepals, petals, stamens and carpels, there is a remarkable amount of morphological diversity. Interestingly, all flowers are specified by the conserved (A)BCE-model. Most of the transcription factors in this model belong to the MADS-domain family. We examined how these transcription factors and their binding sites in the genome evolved, as a first step to elucidate how diversity in flower morphology has been created. We analyzed the evolution of transcription factor binding sites by comparing binding sites of the major floral regulator SEPALLATA3 between two closely related Arabidopsis species, as well as between A. thaliana ecotypes. We found substantial overlap in transcription factor binding profiles between ecotypes, but limited overlap between the related species. We also assessed how transcription factors themselves can change in their properties by analyzing the divergence between paralogs. We examined how the PISTILLATA paralogs in Tarenaya hassleriana diverged, as this species occupies an interesting position in the eudicot phylogeny. We also studied whether divergence of the APETALA3 paralogs in Aquilegia could explain the specification of an additional floral organ in this genus.  In both cases, we conclude that the paralogs diverged from each other in their biochemical properties. In the future, it would be interesting to assess how these changes in transcription factors and their binding sites affect floral regulatory networks and ultimately floral shape.

AB - Although most flowers follow a conserved 'bauplan' consisting of sepals, petals, stamens and carpels, there is a remarkable amount of morphological diversity. Interestingly, all flowers are specified by the conserved (A)BCE-model. Most of the transcription factors in this model belong to the MADS-domain family. We examined how these transcription factors and their binding sites in the genome evolved, as a first step to elucidate how diversity in flower morphology has been created. We analyzed the evolution of transcription factor binding sites by comparing binding sites of the major floral regulator SEPALLATA3 between two closely related Arabidopsis species, as well as between A. thaliana ecotypes. We found substantial overlap in transcription factor binding profiles between ecotypes, but limited overlap between the related species. We also assessed how transcription factors themselves can change in their properties by analyzing the divergence between paralogs. We examined how the PISTILLATA paralogs in Tarenaya hassleriana diverged, as this species occupies an interesting position in the eudicot phylogeny. We also studied whether divergence of the APETALA3 paralogs in Aquilegia could explain the specification of an additional floral organ in this genus.  In both cases, we conclude that the paralogs diverged from each other in their biochemical properties. In the future, it would be interesting to assess how these changes in transcription factors and their binding sites affect floral regulatory networks and ultimately floral shape.

KW - plants

KW - evolution

KW - mads-box proteins

KW - transcription factors

KW - flowers

KW - molecular biology

KW - planten

KW - evolutie

KW - mads-box eiwitten

KW - transcriptiefactoren

KW - bloemen

KW - moleculaire biologie

U2 - 10.18174/421346

DO - 10.18174/421346

M3 - internal PhD, WU

SN - 9789463436700

PB - Wageningen University

CY - Wageningen

ER -