LuxR homolog-linked biosynthetic gene clusters in Proteobacteria

Carolyn A. Brotherton, Marnix H. Medema, E.P. Greenberg

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Microbes are a major source of antibiotics, pharmaceuticals, and other bioactive compounds. The production of many specialized microbial metabolites is encoded in biosynthetic gene clusters (BGCs). A challenge associated with natural product discovery is that many BGCs are not expressed under laboratory growth conditions. Here we report a genome-mining approach to discover BGCs with luxRtype quorum sensing (QS) genes, which code for regulatory proteins that control gene expression. Our results show that BGCs linked to genes coding for LuxR-like proteins are widespread in Proteobacteria. In addition, we show that associations between luxR homolog genes and BGCs have evolved independently many times, with functionally diverse gene clusters. Overall, these clusters may provide a source of new natural products for which there is some understanding about how to elicit production. IMPORTANCE Bacteria biosynthesize specialized metabolites with a variety of ecological functions, including defense against other microbes. Genes that code for specialized metabolite biosynthetic enzymes are frequently clustered together. These BGCs are often regulated by a transcription factor encoded within the cluster itself. These pathway-specific regulators respond to a signal or indirectly through other means of environmental sensing. Many specialized metabolites are not produced under laboratory growth conditions, and one reason for this issue is that laboratory growth media lack environmental cues necessary for BGC expression. Here, we report a bioinformatics study that reveals that BGCs are frequently linked to genes coding for LuxR family QS-responsive transcription factors in the phylum Proteobacteria. The products of these luxR homolog-associated gene clusters may serve as a practical source of bioactive metabolites.
Original languageEnglish
Article numbere00208-17
JournalmSystems
Volume3
Issue number3
DOIs
Publication statusPublished - 26 Jun 2018

Fingerprint

Proteobacteria
Multigene Family
multigene family
Genes
Gene
gene
metabolites
Metabolites
metabolite
Quorum Sensing
quorum sensing
Biological Products
genes
Transcription Factors
transcription factors
Growth
Transcription factors
Transcription Factor
Growth Conditions
microorganisms

Keywords

  • Quorum sensing
  • Secondary metabolism

Cite this

Brotherton, Carolyn A. ; Medema, Marnix H. ; Greenberg, E.P. / LuxR homolog-linked biosynthetic gene clusters in Proteobacteria. In: mSystems. 2018 ; Vol. 3, No. 3.
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LuxR homolog-linked biosynthetic gene clusters in Proteobacteria. / Brotherton, Carolyn A.; Medema, Marnix H.; Greenberg, E.P.

In: mSystems, Vol. 3, No. 3, e00208-17, 26.06.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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