Luciferase detection during stationary phase in Lactococcus lactis

H. Bachmann; F. dos Santos; M. Kleerebezem; J.E.T. van Hylckama Vlieg

doi:10.1128/AEM.02807-06

Luciferase detection during stationary phase in Lactococcus lactis

H. Bachmann, F. dos Santos, M. Kleerebezem, J.E.T. van Hylckama Vlieg

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems

Original language	English
Pages (from-to)	4704-4706
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	14
DOIs	https://doi.org/10.1128/AEM.02807-06
Publication status	Published - 2007

Keywords

gene-expression
bioluminescence
mg1363
operon
mice

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10.1128/AEM.02807-06

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title = "Luciferase detection during stationary phase in Lactococcus lactis",

abstract = "The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems",

keywords = "gene-expression, bioluminescence, mg1363, operon, mice",

author = "H. Bachmann and \{dos Santos\}, F. and M. Kleerebezem and \{van Hylckama Vlieg\}, J.E.T.",

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T1 - Luciferase detection during stationary phase in Lactococcus lactis

AU - Bachmann, H.

AU - dos Santos, F.

AU - Kleerebezem, M.

AU - van Hylckama Vlieg, J.E.T.

N1 - WoS

PY - 2007

Y1 - 2007

N2 - The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems

AB - The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems

KW - gene-expression

KW - bioluminescence

KW - mg1363

KW - operon

KW - mice

U2 - 10.1128/AEM.02807-06

DO - 10.1128/AEM.02807-06

M3 - Article

SN - 0099-2240

VL - 73

SP - 4704

EP - 4706

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 14

ER -

Luciferase detection during stationary phase in Lactococcus lactis

Abstract

Keywords

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