Luciferase detection during stationary phase in Lactococcus lactis

H. Bachmann, F. dos Santos, M. Kleerebezem, J.E.T. van Hylckama Vlieg

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems
Original languageEnglish
Pages (from-to)4704-4706
JournalApplied and Environmental Microbiology
Volume73
Issue number14
DOIs
Publication statusPublished - 2007

Keywords

  • gene-expression
  • bioluminescence
  • mg1363
  • operon
  • mice

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