LPCAT1 controls phosphate homeostasis in a zinc-dependent manner

Mushtak Kisko, Nadia Bouain, Alaeddine Safi, Anna Medici, Robert C. Akkers, David Secco, Gilles Fouret, Gabriel Krouk, Mark G.M. Aarts, Wolfgang Busch, Hatem Rouached*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

All living organisms require a variety of essential elements for their basic biological functions. While the homeostasis of nutrients is highly intertwined, the molecular and genetic mechanisms of these dependencies remain poorly understood. Here, we report a discovery of a molecular pathway that controls phosphate (Pi) accumulation in plants under Zn deficiency. Using genome-wide association studies, we first identified allelic variation of the Lyso- Phosphatidyl Choline (PC) Acyl Transferase 1 (LPCAT1) gene as the key determinant of shoot Pi accumulation under Zn deficiency. We then show that regulatory variation at the LPCAT1 locus contributes significantly to this natural variation and we further demonstrate that the regulation of LPCAT1 expression involves bZIP23 TF, for which we identified a new binding site sequence. Finally, we show that in Zn deficient conditions loss of function of LPCAT1 increases the phospholipid Lyso-Phosphatidyl Choline/Phosphatidyl Choline ratio, the expression of the Pi transporter PHT1;1, and that this leads to shoot Pi accumulation.

Original languageEnglish
Article numbere32077
JournaleLife
Volume7
DOIs
Publication statusPublished - 17 Feb 2018

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    Kisko, M., Bouain, N., Safi, A., Medici, A., Akkers, R. C., Secco, D., ... Rouached, H. (2018). LPCAT1 controls phosphate homeostasis in a zinc-dependent manner. eLife, 7, [e32077]. https://doi.org/10.7554/eLife.32077