Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1963-1967 |
| Journal | Journal of General Virology |
| Volume | 98 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2017 |
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Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats. / Mazza, M.; Guglielmetti, Chiara; Ingravalle, Francesco; Brusadore, Sonia; Langeveld, J.P.M.; Ekateriniadou, Loukia V.; Andreoletti, O.; Casalone, C.; Acutis, P.L.
In: Journal of General Virology, Vol. 98, No. 7, 2017, p. 1963-1967.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats
AU - Mazza, M.
AU - Guglielmetti, Chiara
AU - Ingravalle, Francesco
AU - Brusadore, Sonia
AU - Langeveld, J.P.M.
AU - Ekateriniadou, Loukia V.
AU - Andreoletti, O.
AU - Casalone, C.
AU - Acutis, P.L.
PY - 2017
Y1 - 2017
N2 - The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.
AB - The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.
U2 - 10.1099/jgv.0.000843
DO - 10.1099/jgv.0.000843
M3 - Article
VL - 98
SP - 1963
EP - 1967
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 7
ER -