Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats

M. Mazza; Chiara Guglielmetti; Francesco Ingravalle; Sonia Brusadore; J.P.M. Langeveld; Loukia V. Ekateriniadou; O. Andreoletti; C. Casalone; P.L. Acutis

doi:10.1099/jgv.0.000843

Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats

M. Mazza^*, Chiara Guglielmetti, Francesco Ingravalle, Sonia Brusadore, J.P.M. Langeveld, Loukia V. Ekateriniadou, O. Andreoletti, C. Casalone, P.L. Acutis

^*Corresponding author for this work

Bacteriology, Host Pathogen Interaction & Diagnostics

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.

Original language	English
Pages (from-to)	1963-1967
Journal	Journal of General Virology
Volume	98
Issue number	7
DOIs	https://doi.org/10.1099/jgv.0.000843
Publication status	Published - 2017

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@article{3f94963e2f0445af876e5d6e296c2c30,

title = "Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats",

abstract = "The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.",

author = "M. Mazza and Chiara Guglielmetti and Francesco Ingravalle and Sonia Brusadore and J.P.M. Langeveld and Ekateriniadou, {Loukia V.} and O. Andreoletti and C. Casalone and P.L. Acutis",

year = "2017",

doi = "10.1099/jgv.0.000843",

language = "English",

volume = "98",

pages = "1963--1967",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Microbiology Society",

number = "7",

}

Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats. / Mazza, M.; Guglielmetti, Chiara; Ingravalle, Francesco; Brusadore, Sonia; Langeveld, J.P.M.; Ekateriniadou, Loukia V.; Andreoletti, O.; Casalone, C.; Acutis, P.L.

In: Journal of General Virology, Vol. 98, No. 7, 2017, p. 1963-1967.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats

AU - Mazza, M.

AU - Guglielmetti, Chiara

AU - Ingravalle, Francesco

AU - Brusadore, Sonia

AU - Langeveld, J.P.M.

AU - Ekateriniadou, Loukia V.

AU - Andreoletti, O.

AU - Casalone, C.

AU - Acutis, P.L.

PY - 2017

Y1 - 2017

N2 - The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.

AB - The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.

U2 - 10.1099/jgv.0.000843

DO - 10.1099/jgv.0.000843

M3 - Article

VL - 98

SP - 1963

EP - 1967

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 7

ER -