Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats

M. Mazza, Chiara Guglielmetti, Francesco Ingravalle, Sonia Brusadore, J.P.M. Langeveld, Loukia V. Ekateriniadou, O. Andreoletti, C. Casalone, P.L. Acutis

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2 Citations (Scopus)

Abstract

The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.
Original languageEnglish
Pages (from-to)1963-1967
JournalJournal of General Virology
Volume98
Issue number7
DOIs
Publication statusPublished - 2017

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Scrapie
Goats
Peptide Hydrolases
Immunoblotting
Codon
Lysine
Monoclonal Antibodies
Mutation

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Mazza, M., Guglielmetti, C., Ingravalle, F., Brusadore, S., Langeveld, J. P. M., Ekateriniadou, L. V., ... Acutis, P. L. (2017). Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats. Journal of General Virology, 98(7), 1963-1967. https://doi.org/10.1099/jgv.0.000843
Mazza, M. ; Guglielmetti, Chiara ; Ingravalle, Francesco ; Brusadore, Sonia ; Langeveld, J.P.M. ; Ekateriniadou, Loukia V. ; Andreoletti, O. ; Casalone, C. ; Acutis, P.L. / Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats. In: Journal of General Virology. 2017 ; Vol. 98, No. 7. pp. 1963-1967.
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title = "Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats",
abstract = "The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.",
author = "M. Mazza and Chiara Guglielmetti and Francesco Ingravalle and Sonia Brusadore and J.P.M. Langeveld and Ekateriniadou, {Loukia V.} and O. Andreoletti and C. Casalone and P.L. Acutis",
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Mazza, M, Guglielmetti, C, Ingravalle, F, Brusadore, S, Langeveld, JPM, Ekateriniadou, LV, Andreoletti, O, Casalone, C & Acutis, PL 2017, 'Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats', Journal of General Virology, vol. 98, no. 7, pp. 1963-1967. https://doi.org/10.1099/jgv.0.000843

Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats. / Mazza, M.; Guglielmetti, Chiara; Ingravalle, Francesco; Brusadore, Sonia; Langeveld, J.P.M.; Ekateriniadou, Loukia V.; Andreoletti, O.; Casalone, C.; Acutis, P.L.

In: Journal of General Virology, Vol. 98, No. 7, 2017, p. 1963-1967.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats

AU - Mazza, M.

AU - Guglielmetti, Chiara

AU - Ingravalle, Francesco

AU - Brusadore, Sonia

AU - Langeveld, J.P.M.

AU - Ekateriniadou, Loukia V.

AU - Andreoletti, O.

AU - Casalone, C.

AU - Acutis, P.L.

PY - 2017

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AB - The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.

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M3 - Article

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