Loss of aphid transmissibility of plum pox virus isolates

I. Kamenova, H. Lohuis, D. Peters

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The aphid transmissibility of seven Plum pox virus (PPV) isolates and the amino acid sequences of their coat proteins were analysed Two aphid transmissible isolates PPV-A and PPV-P contained the DAG amino triplet, while DAL or NAG replaced this triplet in the coat proteins of non-aphid transmissible isolates. In addition, a sequence of 15 or 17 amino acids was deleted in the coat proteins of PPV-P48, PPV-P45 and PPV-II/7. Helper components with Mr. of 52 kDa were successfully purified from N. benthamiana plants inoculated with aphid transmissible and non-aphid transmissible isolates. The helper components of two coat-proteins-defective isolates mediated aphid transmission of non-coat-protein defective isolates, indicating that the helper components were biologically active.
Original languageEnglish
Pages (from-to)48-54
JournalBiotechnology and Biotechnological Equipment
Volume16
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • complete nucleotide-sequence
  • yellow mosaic-virus
  • y helper component
  • plant-viruses
  • protein gene
  • potyvirus
  • rna
  • strain
  • identification
  • purification

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