Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection

Aldrik H. Velders, Cor Schoen, Vittorio Saggiomo*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

Objective: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. Results: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field.
Original languageEnglish
Article number3197
JournalBMC Research Notes
Volume11
DOIs
Publication statusPublished - 1 Feb 2018

Keywords

  • Arduino instruments
  • Loop mediated isothermal amplification
  • Nucleic acid detection
  • Portable

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