Long-term probiotic intervention affects B cell differentiation in bone marrow of fast ageing Ercc1-/¿7 mice

A.A. van Beek; B. Sovran; B. Meijer; P. de Vos; J.J.H. Hoeijmakers; P.J. Leenen; R.W. Hendriks; H.F.J. Savelkoul

Long-term probiotic intervention affects B cell differentiation in bone marrow of fast ageing Ercc1-/¿7 mice

A.A. van Beek
, B. Sovran
, B. Meijer
, P. de Vos
, J.J.H. Hoeijmakers
, P.J. Leenen
, R.W. Hendriks
, H.F.J. Savelkoul

Research output: Contribution to conference › Abstract

Abstract

Gut microbiota and immunity are related and alter upon ageing. Therefore, we investigated the effects of probiotics on maturation and function of ageing B cells. We used fast ageing Ercc1-/¿7 mice (median lifespan 20 weeks) and wild-type mice to study the effect of probiotics on the ageing immune system. Mice were supplemented the probiotic strains L. plantarum WCFS1, L. casei BL23 and B. breve DSM20213 by gavage for 10 weeks. At 16 weeks of age, the B cell composition of various organs was measured by multicolour flow cytometry. Immune competence was evaluated by stimulating spleen and bone marrow (BM) cells with several ligands (e.g. LPS). B cell frequencies in particular decreased in the BM after L. casei treatment in Ercc1-/¿7 (15 vs. 27%; p

Original language	English
Publication status	Published - 2014
Event	50th Anniversary Dutch Society for Immunologie (NVVI), Kaatsheuvel NL - Duration: 17 Dec 2014 → 19 Dec 2014

Conference/symposium

Conference/symposium	50th Anniversary Dutch Society for Immunologie (NVVI), Kaatsheuvel NL
Period	17/12/14 → 19/12/14

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@conference{c5435bac785b404491f2171cf6e91446,

title = "Long-term probiotic intervention affects B cell differentiation in bone marrow of fast ageing Ercc1-/¿7 mice",

abstract = "Gut microbiota and immunity are related and alter upon ageing. Therefore, we investigated the effects of probiotics on maturation and function of ageing B cells. We used fast ageing Ercc1-/¿7 mice (median lifespan 20 weeks) and wild-type mice to study the effect of probiotics on the ageing immune system. Mice were supplemented the probiotic strains L. plantarum WCFS1, L. casei BL23 and B. breve DSM20213 by gavage for 10 weeks. At 16 weeks of age, the B cell composition of various organs was measured by multicolour flow cytometry. Immune competence was evaluated by stimulating spleen and bone marrow (BM) cells with several ligands (e.g. LPS). B cell frequencies in particular decreased in the BM after L. casei treatment in Ercc1-/¿7 (15 vs. 27\%; p",

author = "\{van Beek\}, A.A. and B. Sovran and B. Meijer and \{de Vos\}, P. and J.J.H. Hoeijmakers and P.J. Leenen and R.W. Hendriks and H.F.J. Savelkoul",

year = "2014",

language = "English",

note = "50th Anniversary Dutch Society for Immunologie (NVVI), Kaatsheuvel NL ; Conference date: 17-12-2014 Through 19-12-2014",

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TY - CONF

T1 - Long-term probiotic intervention affects B cell differentiation in bone marrow of fast ageing Ercc1-/¿7 mice

AU - van Beek, A.A.

AU - Sovran, B.

AU - Meijer, B.

AU - de Vos, P.

AU - Hoeijmakers, J.J.H.

AU - Leenen, P.J.

AU - Hendriks, R.W.

AU - Savelkoul, H.F.J.

PY - 2014

Y1 - 2014

N2 - Gut microbiota and immunity are related and alter upon ageing. Therefore, we investigated the effects of probiotics on maturation and function of ageing B cells. We used fast ageing Ercc1-/¿7 mice (median lifespan 20 weeks) and wild-type mice to study the effect of probiotics on the ageing immune system. Mice were supplemented the probiotic strains L. plantarum WCFS1, L. casei BL23 and B. breve DSM20213 by gavage for 10 weeks. At 16 weeks of age, the B cell composition of various organs was measured by multicolour flow cytometry. Immune competence was evaluated by stimulating spleen and bone marrow (BM) cells with several ligands (e.g. LPS). B cell frequencies in particular decreased in the BM after L. casei treatment in Ercc1-/¿7 (15 vs. 27%; p

AB - Gut microbiota and immunity are related and alter upon ageing. Therefore, we investigated the effects of probiotics on maturation and function of ageing B cells. We used fast ageing Ercc1-/¿7 mice (median lifespan 20 weeks) and wild-type mice to study the effect of probiotics on the ageing immune system. Mice were supplemented the probiotic strains L. plantarum WCFS1, L. casei BL23 and B. breve DSM20213 by gavage for 10 weeks. At 16 weeks of age, the B cell composition of various organs was measured by multicolour flow cytometry. Immune competence was evaluated by stimulating spleen and bone marrow (BM) cells with several ligands (e.g. LPS). B cell frequencies in particular decreased in the BM after L. casei treatment in Ercc1-/¿7 (15 vs. 27%; p

M3 - Abstract

T2 - 50th Anniversary Dutch Society for Immunologie (NVVI), Kaatsheuvel NL

Y2 - 17 December 2014 through 19 December 2014

ER -

Long-term probiotic intervention affects B cell differentiation in bone marrow of fast ageing Ercc1-/¿7 mice

Abstract

Conference/symposium

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